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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Desulfovibrio vulgaris Octanoyltransferase (lipB) CSB-YP751371DDH
CSB-EP751371DDH
CSB-BP751371DDH
CSB-MP751371DDH
CSB-EP751371DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Shikimate kinase (aroK) CSB-YP751372DDH
CSB-EP751372DDH
CSB-BP751372DDH
CSB-MP751372DDH
CSB-EP751372DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Uridylate kinase (pyrH) CSB-YP751373DDH
CSB-EP751373DDH
CSB-BP751373DDH
CSB-MP751373DDH
CSB-EP751373DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris UPF0102 protein DVU_0833 (DVU_0833) CSB-YP751374DDH
CSB-EP751374DDH
CSB-BP751374DDH
CSB-MP751374DDH
CSB-EP751374DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Cobyric acid synthase (cobQ), partial CSB-YP751375DDH
CSB-EP751375DDH
CSB-BP751375DDH
CSB-MP751375DDH
CSB-EP751375DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris UvrABC system protein C (uvrC), partial CSB-YP751376DDH
CSB-EP751376DDH
CSB-BP751376DDH
CSB-MP751376DDH
CSB-EP751376DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris ATP synthase epsilon chain (atpC) CSB-YP751377DDH
CSB-EP751377DDH
CSB-BP751377DDH
CSB-MP751377DDH
CSB-EP751377DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Elongation factor 4 (lepA), partial CSB-YP751378DDH
CSB-EP751378DDH
CSB-BP751378DDH
CSB-MP751378DDH
CSB-EP751378DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 30S ribosomal protein S15 (rpsO) CSB-YP751379DDH
CSB-EP751379DDH
CSB-BP751379DDH
CSB-MP751379DDH
CSB-EP751379DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP751380DDH1
CSB-EP751380DDH1
CSB-BP751380DDH1
CSB-MP751380DDH1
CSB-EP751380DDH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP751381TNS
CSB-EP751381TNS
CSB-BP751381TNS
CSB-MP751381TNS
CSB-EP751381TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 50S ribosomal protein L1 (rplA) CSB-YP751382TNS
CSB-EP751382TNS
CSB-BP751382TNS
CSB-MP751382TNS
CSB-EP751382TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Phosphate import ATP-binding protein PstB (pstB) CSB-YP751383TNS
CSB-EP751383TNS
CSB-BP751383TNS
CSB-MP751383TNS
CSB-EP751383TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus UPF0042 nucleotide-binding protein TT_C1664 (TT_C1664) CSB-YP751384TNS
CSB-EP751384TNS
CSB-BP751384TNS
CSB-MP751384TNS
CSB-EP751384TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Chromosomal replication initiator protein DnaA (dnaA) CSB-YP751385TNS
CSB-EP751385TNS
CSB-BP751385TNS
CSB-MP751385TNS
CSB-EP751385TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP751386TNS
CSB-EP751386TNS
CSB-BP751386TNS
CSB-MP751386TNS
CSB-EP751386TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Probable tRNA sulfurtransferase (thiI) CSB-YP751387TNS
CSB-EP751387TNS
CSB-BP751387TNS
CSB-MP751387TNS
CSB-EP751387TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 50S ribosomal protein L14 (rplN) CSB-YP751388TNS
CSB-EP751388TNS
CSB-BP751388TNS
CSB-MP751388TNS
CSB-EP751388TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 50S ribosomal protein L6 (rplF) CSB-YP751389TNS
CSB-EP751389TNS
CSB-BP751389TNS
CSB-MP751389TNS
CSB-EP751389TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Non-canonical purine NTP pyrophosphatase (TT_C1290) CSB-YP751390TNS
CSB-EP751390TNS
CSB-BP751390TNS
CSB-MP751390TNS
CSB-EP751390TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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