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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rabbit Elongation factor 1-alpha 2 (EEF1A2) CSB-YP751271RB
CSB-EP751271RB
CSB-BP751271RB
CSB-MP751271RB
CSB-EP751271RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Importin subunit alpha-1a (Os01g0253300, LOC_Os01g14950), partial CSB-YP751272OFG
CSB-EP751272OFG
CSB-BP751272OFG
CSB-MP751272OFG
CSB-EP751272OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Ribonuclease P protein component (rnpA) CSB-YP751273LAAD
CSB-EP751273LAAD
CSB-BP751273LAAD
CSB-MP751273LAAD
CSB-EP751273LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b L-rhamnose isomerase (rhaA) CSB-YP751274LAAD
CSB-EP751274LAAD
CSB-BP751274LAAD
CSB-MP751274LAAD
CSB-EP751274LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b tRNA modification GTPase MnmE (mnmE) CSB-YP751275LAAD
CSB-EP751275LAAD
CSB-BP751275LAAD
CSB-MP751275LAAD
CSB-EP751275LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Glutathione biosynthesis bifunctional protein gshAB (gshAB), partial CSB-YP751276LAAD
CSB-EP751276LAAD
CSB-BP751276LAAD
CSB-MP751276LAAD
CSB-EP751276LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Potassium-transporting ATPase B chain (kdpB), partial CSB-YP751277LAAD1
CSB-EP751277LAAD1
CSB-BP751277LAAD1
CSB-MP751277LAAD1
CSB-EP751277LAAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 50S ribosomal protein L4 (rplD) CSB-YP751278LAAD
CSB-EP751278LAAD
CSB-BP751278LAAD
CSB-MP751278LAAD
CSB-EP751278LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 50S ribosomal protein L22 (rplV) CSB-YP751279LAAD
CSB-EP751279LAAD
CSB-BP751279LAAD
CSB-MP751279LAAD
CSB-EP751279LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 30S ribosomal protein S5 (rpsE) CSB-YP751280LAAD
CSB-EP751280LAAD
CSB-BP751280LAAD
CSB-MP751280LAAD
CSB-EP751280LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 50S ribosomal protein L13 (rplM) CSB-YP751281LAAD
CSB-EP751281LAAD
CSB-BP751281LAAD
CSB-MP751281LAAD
CSB-EP751281LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Arginine--tRNA ligase (argS), partial CSB-YP751282LAAD
CSB-EP751282LAAD
CSB-BP751282LAAD
CSB-MP751282LAAD
CSB-EP751282LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Uracil phosphoribosyltransferase (upp) CSB-YP751283LAAD
CSB-EP751283LAAD
CSB-BP751283LAAD
CSB-MP751283LAAD
CSB-EP751283LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Cardiolipin synthase (cls), partial CSB-YP751284LAAD1
CSB-EP751284LAAD1
CSB-BP751284LAAD1
CSB-MP751284LAAD1
CSB-EP751284LAAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b UvrABC system protein A (uvrA), partial CSB-YP751285LAAD
CSB-EP751285LAAD
CSB-BP751285LAAD
CSB-MP751285LAAD
CSB-EP751285LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Enolase (eno) CSB-YP751286LAAD
CSB-EP751286LAAD
CSB-BP751286LAAD
CSB-MP751286LAAD
CSB-EP751286LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Fumarate hydratase class II (fumC) CSB-YP751287LAAD
CSB-EP751287LAAD
CSB-BP751287LAAD
CSB-MP751287LAAD
CSB-EP751287LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Foldase protein prsA 2 (prsA2) CSB-YP751288LAAD
CSB-EP751288LAAD
CSB-BP751288LAAD
CSB-MP751288LAAD
CSB-EP751288LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Regulatory protein spx (spxA) CSB-YP751289LAAD
CSB-EP751289LAAD
CSB-BP751289LAAD
CSB-MP751289LAAD
CSB-EP751289LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Redox-sensing transcriptional repressor rex (rex) CSB-YP751290LAAD
CSB-EP751290LAAD
CSB-BP751290LAAD
CSB-MP751290LAAD
CSB-EP751290LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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