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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Listeria monocytogenes serotype 4b Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP751331LAAD
CSB-EP751331LAAD
CSB-BP751331LAAD
CSB-MP751331LAAD
CSB-EP751331LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Shikimate dehydrogenase (aroE) CSB-YP751332LAAD
CSB-EP751332LAAD
CSB-BP751332LAAD
CSB-MP751332LAAD
CSB-EP751332LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Heme-degrading monooxygenase isdG (isdG) CSB-YP751333LAAD
CSB-EP751333LAAD
CSB-BP751333LAAD
CSB-MP751333LAAD
CSB-EP751333LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Hydroxyethylthiazole kinase (thiM) CSB-YP751334LAAD
CSB-EP751334LAAD
CSB-BP751334LAAD
CSB-MP751334LAAD
CSB-EP751334LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 50S ribosomal protein L7/L12 (rplL) CSB-YP751335LAAD
CSB-EP751335LAAD
CSB-BP751335LAAD
CSB-MP751335LAAD
CSB-EP751335LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Glutamate--tRNA ligase (gltX) CSB-YP751336LAAD
CSB-EP751336LAAD
CSB-BP751336LAAD
CSB-MP751336LAAD
CSB-EP751336LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Type III pantothenate kinase (coaX) CSB-YP751337LAAD
CSB-EP751337LAAD
CSB-BP751337LAAD
CSB-MP751337LAAD
CSB-EP751337LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b UPF0145 protein LMOf2365_0219 (LMOf2365_0219) CSB-YP751338LAAD
CSB-EP751338LAAD
CSB-BP751338LAAD
CSB-MP751338LAAD
CSB-EP751338LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP751339LAAD
CSB-EP751339LAAD
CSB-BP751339LAAD
CSB-MP751339LAAD
CSB-EP751339LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Adenylosuccinate synthetase (purA) CSB-YP751340LAAD
CSB-EP751340LAAD
CSB-BP751340LAAD
CSB-MP751340LAAD
CSB-EP751340LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 30S ribosomal protein S6 (rpsF) CSB-YP751341LAAD
CSB-EP751341LAAD
CSB-BP751341LAAD
CSB-MP751341LAAD
CSB-EP751341LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Putative agmatine deiminase 1 (aguA1) CSB-YP751342LAAD
CSB-EP751342LAAD
CSB-BP751342LAAD
CSB-MP751342LAAD
CSB-EP751342LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Zinc resistance-associated protein homolog (DVU_3384) CSB-YP751343DDH
CSB-EP751343DDH
CSB-BP751343DDH
CSB-MP751343DDH
CSB-EP751343DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP751344DDH1
CSB-EP751344DDH1
CSB-BP751344DDH1
CSB-MP751344DDH1
CSB-EP751344DDH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP751345DDH
CSB-EP751345DDH
CSB-BP751345DDH
CSB-MP751345DDH
CSB-EP751345DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Probable chemoreceptor glutamine deamidase CheD (cheD) CSB-YP751346DDH
CSB-EP751346DDH
CSB-BP751346DDH
CSB-MP751346DDH
CSB-EP751346DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP751347DDH
CSB-EP751347DDH
CSB-BP751347DDH
CSB-MP751347DDH
CSB-EP751347DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP751348DDH
CSB-EP751348DDH
CSB-BP751348DDH
CSB-MP751348DDH
CSB-EP751348DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Ribonuclease Y (rny), partial CSB-YP751349DDH
CSB-EP751349DDH
CSB-BP751349DDH
CSB-MP751349DDH
CSB-EP751349DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Chaperone protein htpG (htpG), partial CSB-YP751350DDH
CSB-EP751350DDH
CSB-BP751350DDH
CSB-MP751350DDH
CSB-EP751350DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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