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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Translation initiation factor IF-2 (infB), partial CSB-YP753396SKW
CSB-EP753396SKW
CSB-BP753396SKW
CSB-MP753396SKW
CSB-EP753396SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S2 (rpsB) CSB-YP753397SKW
CSB-EP753397SKW
CSB-BP753397SKW
CSB-MP753397SKW
CSB-EP753397SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribosome maturation factor RimM (rimM) CSB-YP753398SKW
CSB-EP753398SKW
CSB-BP753398SKW
CSB-MP753398SKW
CSB-EP753398SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Malonyl CoA-acyl carrier protein transacylase (fabD) CSB-YP753399SKW
CSB-EP753399SKW
CSB-BP753399SKW
CSB-MP753399SKW
CSB-EP753399SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP753400SKW
CSB-EP753400SKW
CSB-BP753400SKW
CSB-MP753400SKW
CSB-EP753400SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Aspartate carbamoyltransferase (pyrB) CSB-YP753401SKW
CSB-EP753401SKW
CSB-BP753401SKW
CSB-MP753401SKW
CSB-EP753401SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Cell division protein FtsZ (ftsZ) CSB-YP753402SKW
CSB-EP753402SKW
CSB-BP753402SKW
CSB-MP753402SKW
CSB-EP753402SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Fibrinogen-binding protein (fib) CSB-YP753403SKW
CSB-EP753403SKW
CSB-BP753403SKW
CSB-MP753403SKW
CSB-EP753403SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glutamate racemase (murI) CSB-YP753404SKW
CSB-EP753404SKW
CSB-BP753404SKW
CSB-MP753404SKW
CSB-EP753404SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP753405SKW
CSB-EP753405SKW
CSB-BP753405SKW
CSB-MP753405SKW
CSB-EP753405SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Iron-regulated surface determinant protein B (isdB), partial CSB-YP753406SKW
CSB-EP753406SKW
CSB-BP753406SKW
CSB-MP753406SKW
CSB-EP753406SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Dihydrolipoyl dehydrogenase (pdhD) CSB-YP753407SKW
CSB-EP753407SKW
CSB-BP753407SKW
CSB-MP753407SKW
CSB-EP753407SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Amidophosphoribosyltransferase (purF) CSB-YP753408SKW
CSB-EP753408SKW
CSB-BP753408SKW
CSB-MP753408SKW
CSB-EP753408SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glutamyl endopeptidase (sspA) CSB-YP753409SKW
CSB-EP753409SKW
CSB-BP753409SKW
CSB-MP753409SKW
CSB-EP753409SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine protease htrA-like (SAS0955), partial CSB-YP753410SKW1
CSB-EP753410SKW1
CSB-BP753410SKW1
CSB-MP753410SKW1
CSB-EP753410SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0477 protein SAS0884 (SAS0884) CSB-YP753411SKW
CSB-EP753411SKW
CSB-BP753411SKW
CSB-MP753411SKW
CSB-EP753411SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Chaperone protein ClpB (clpB), partial CSB-YP753412SKW
CSB-EP753412SKW
CSB-BP753412SKW
CSB-MP753412SKW
CSB-EP753412SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Inactive signal peptidase IA (spsA), partial CSB-YP753413SKW
CSB-EP753413SKW
CSB-BP753413SKW
CSB-MP753413SKW
CSB-EP753413SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Na (+)/H (+) antiporter subunit B1 (mnhB1), partial CSB-YP753414SKW1
CSB-EP753414SKW1
CSB-BP753414SKW1
CSB-MP753414SKW1
CSB-EP753414SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable cysteine desulfurase (csd) CSB-YP753415SKW
CSB-EP753415SKW
CSB-BP753415SKW
CSB-MP753415SKW
CSB-EP753415SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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