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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bdellovibrio bacteriovorus DNA ligase (ligA), partial CSB-YP753997BFP
CSB-EP753997BFP
CSB-BP753997BFP
CSB-MP753997BFP
CSB-EP753997BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Peptidyl-tRNA hydrolase (pth) CSB-YP753998MLS
CSB-EP753998MLS
CSB-BP753998MLS
CSB-MP753998MLS
CSB-EP753998MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Uracil phosphoribosyltransferase (upp) CSB-YP753999MLS
CSB-EP753999MLS
CSB-BP753999MLS
CSB-MP753999MLS
CSB-EP753999MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC SsrA-binding protein (smpB) CSB-YP754000MLS
CSB-EP754000MLS
CSB-BP754000MLS
CSB-MP754000MLS
CSB-EP754000MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Glucosamine-6-phosphate deaminase (nagB) CSB-YP754001MLS
CSB-EP754001MLS
CSB-BP754001MLS
CSB-MP754001MLS
CSB-EP754001MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 50S ribosomal protein L2 (rplB) CSB-YP754002MLS
CSB-EP754002MLS
CSB-BP754002MLS
CSB-MP754002MLS
CSB-EP754002MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 50S ribosomal protein L18 (rplR) CSB-YP754003MLS
CSB-EP754003MLS
CSB-BP754003MLS
CSB-MP754003MLS
CSB-EP754003MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC tRNA pseudouridine synthase A (truA) CSB-YP754004MLS
CSB-EP754004MLS
CSB-BP754004MLS
CSB-MP754004MLS
CSB-EP754004MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Protein grpE (grpE) CSB-YP754005MLS
CSB-EP754005MLS
CSB-BP754005MLS
CSB-MP754005MLS
CSB-EP754005MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Isoleucine--tRNA ligase (ileS), partial CSB-YP754006MLS
CSB-EP754006MLS
CSB-BP754006MLS
CSB-MP754006MLS
CSB-EP754006MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 50S ribosomal protein L28 (rpmB) CSB-YP754007MLS
CSB-EP754007MLS
CSB-BP754007MLS
CSB-MP754007MLS
CSB-EP754007MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Elongation factor P (efp) CSB-YP754008MLS
CSB-EP754008MLS
CSB-BP754008MLS
CSB-MP754008MLS
CSB-EP754008MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC GTPase obg (obg) CSB-YP754009MLS
CSB-EP754009MLS
CSB-BP754009MLS
CSB-MP754009MLS
CSB-EP754009MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Ribonuclease Y (rny), partial CSB-YP754010MLS
CSB-EP754010MLS
CSB-BP754010MLS
CSB-MP754010MLS
CSB-EP754010MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Aspartate--tRNA ligase (aspS), partial CSB-YP754011MLS
CSB-EP754011MLS
CSB-BP754011MLS
CSB-MP754011MLS
CSB-EP754011MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC UvrABC system protein C (uvrC), partial CSB-YP754012MLS
CSB-EP754012MLS
CSB-BP754012MLS
CSB-MP754012MLS
CSB-EP754012MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 50S ribosomal protein L35 (rpmI) CSB-YP754013MLS
CSB-EP754013MLS
CSB-BP754013MLS
CSB-MP754013MLS
CSB-EP754013MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Probable endonuclease 4 (nfo) CSB-YP754014MLS
CSB-EP754014MLS
CSB-BP754014MLS
CSB-MP754014MLS
CSB-EP754014MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Lysine--tRNA ligase (lysS) CSB-YP754015MLS
CSB-EP754015MLS
CSB-BP754015MLS
CSB-MP754015MLS
CSB-EP754015MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Chromosomal replication initiator protein DnaA (dnaA) CSB-YP754016MLS
CSB-EP754016MLS
CSB-BP754016MLS
CSB-MP754016MLS
CSB-EP754016MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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