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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bovine Lactosylceramide alpha-2,3-sialyltransferase (ST3GAL5), partial CSB-YP754599BO
CSB-EP754599BO
CSB-BP754599BO
CSB-MP754599BO
CSB-EP754599BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Tripeptidyl-peptidase sed1 (sed1) CSB-YP754600NGS
CSB-EP754600NGS
CSB-BP754600NGS
CSB-MP754600NGS
CSB-EP754600NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse A-kinase anchor protein 9 (Akap9), partial CSB-YP754601MO
CSB-EP754601MO
CSB-BP754601MO
CSB-MP754601MO
CSB-EP754601MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Signal transducer and activator of transcription B (dstB), partial CSB-YP754602DKK
CSB-EP754602DKK
CSB-BP754602DKK
CSB-MP754602DKK
CSB-EP754602DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Tripeptidyl-peptidase sed2 (sed2) CSB-YP754603NGS
CSB-EP754603NGS
CSB-BP754603NGS
CSB-MP754603NGS
CSB-EP754603NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Biotin synthase (bioB) CSB-YP754604BQB
CSB-EP754604BQB
CSB-BP754604BQB
CSB-MP754604BQB
CSB-EP754604BQB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uromyces fabae Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDH2), partial CSB-YP754605UBN
CSB-EP754605UBN
CSB-BP754605UBN
CSB-MP754605UBN
CSB-EP754605UBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus filamentous virus 1 Putative transmembrane protein ORF63 (ORF63), partial CSB-YP754606AFAE
CSB-EP754606AFAE
CSB-BP754606AFAE
CSB-MP754606AFAE
CSB-EP754606AFAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus filamentous virus 1 Uncharacterized protein ORF307 (ORF307) CSB-YP754607AFAE
CSB-EP754607AFAE
CSB-BP754607AFAE
CSB-MP754607AFAE
CSB-EP754607AFAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus filamentous virus 1 Uncharacterized protein ORF137 (ORF137) CSB-YP754608AFAE
CSB-EP754608AFAE
CSB-BP754608AFAE
CSB-MP754608AFAE
CSB-EP754608AFAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus filamentous virus 1 Uncharacterized protein ORF223 (ORF223) CSB-YP754609AFAE
CSB-EP754609AFAE
CSB-BP754609AFAE
CSB-MP754609AFAE
CSB-EP754609AFAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus filamentous virus 1 Uncharacterized protein ORF135 (ORF135) CSB-YP754610AFAE
CSB-EP754610AFAE
CSB-BP754610AFAE
CSB-MP754610AFAE
CSB-EP754610AFAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus filamentous virus 1 Uncharacterized protein ORF72 (ORF72) CSB-YP754611AFAE
CSB-EP754611AFAE
CSB-BP754611AFAE
CSB-MP754611AFAE
CSB-EP754611AFAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus filamentous virus 1 Uncharacterized protein ORF150 (ORF150) CSB-YP754612AFAE
CSB-EP754612AFAE
CSB-BP754612AFAE
CSB-MP754612AFAE
CSB-EP754612AFAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Linear gramicidin synthase subunit B (lgrB), partial CSB-YP754613BWM
CSB-EP754613BWM
CSB-BP754613BWM
CSB-MP754613BWM
CSB-EP754613BWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Superoxide dismutase [Cu-Zn] (sod1) CSB-YP754614BVC
CSB-EP754614BVC
CSB-BP754614BVC
CSB-MP754614BVC
CSB-EP754614BVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monodon monoceros NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP754615MHW1
CSB-EP754615MHW1
CSB-BP754615MHW1
CSB-MP754615MHW1
CSB-EP754615MHW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Platanista minor NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP754616PHAM1
CSB-EP754616PHAM1
CSB-BP754616PHAM1
CSB-MP754616PHAM1
CSB-EP754616PHAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Cyclic AMP-responsive element-binding protein 3-like protein 2 (CREB3L2), partial CSB-YP754617HU
CSB-BP754617HU
CSB-MP754617HU
CSB-EP754617HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spodoptera frugiperda Allatotropin CSB-YP754618FKQ
CSB-EP754618FKQ
CSB-BP754618FKQ
CSB-MP754618FKQ
CSB-EP754618FKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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