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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xenopus tropicalis THO complex subunit 7 homolog (thoc7) CSB-YP754197XBF
CSB-EP754197XBF
CSB-BP754197XBF
CSB-MP754197XBF
CSB-EP754197XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2 (Alkbh2) CSB-YP754198MO
CSB-EP754198MO
CSB-BP754198MO
CSB-MP754198MO
CSB-EP754198MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Calcium uptake protein 1, mitochondrial (Micu1), partial CSB-YP754199RA
CSB-EP754199RA
CSB-BP754199RA
CSB-MP754199RA
CSB-EP754199RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Complement C1s subcomponent (C1s) CSB-YP754200RA
CSB-EP754200RA
CSB-BP754200RA
CSB-MP754200RA
CSB-EP754200RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat TRPM8 channel-associated factor 3 (Tcaf3), partial CSB-YP754201RA
CSB-EP754201RA
CSB-BP754201RA
CSB-MP754201RA
CSB-EP754201RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Ragulator complex protein LAMTOR1 (Lamtor1) CSB-YP754202RA
CSB-EP754202RA
CSB-BP754202RA
CSB-MP754202RA
CSB-EP754202RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Glycosyltransferase-like protein LARGE2 (Gyltl1b), partial CSB-YP754203RA
CSB-EP754203RA
CSB-BP754203RA
CSB-MP754203RA
CSB-EP754203RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein CNPPD1 (Cnppd1), partial CSB-YP754204RA1
CSB-EP754204RA1
CSB-BP754204RA1
CSB-MP754204RA1
CSB-EP754204RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Probable protein BRICK1-A (brk1-a) CSB-YP754205XBE
CSB-EP754205XBE
CSB-BP754205XBE
CSB-MP754205XBE
CSB-EP754205XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Hydroxysteroid 11-beta-dehydrogenase 1-like protein A (hsd11b1l-a) CSB-YP754206XBE
CSB-EP754206XBE
CSB-BP754206XBE
CSB-MP754206XBE
CSB-EP754206XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Methyltransferase-like protein 20 (Mettl20) CSB-YP754207RA
CSB-EP754207RA
CSB-BP754207RA
CSB-MP754207RA
CSB-EP754207RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse tRNA-splicing endonuclease subunit Sen2 (Tsen2) CSB-YP754208MO
CSB-EP754208MO
CSB-BP754208MO
CSB-MP754208MO
CSB-EP754208MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog (litaf) CSB-YP754209XBF
CSB-EP754209XBF
CSB-BP754209XBF
CSB-MP754209XBF
CSB-EP754209XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis AP-2 complex subunit mu (ap2m1) CSB-YP754210XBF
CSB-EP754210XBF
CSB-BP754210XBF
CSB-MP754210XBF
CSB-EP754210XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Adenylosuccinate synthetase isozyme 2 A (adss-a) CSB-YP754211XBF
CSB-EP754211XBF
CSB-BP754211XBF
CSB-MP754211XBF
CSB-EP754211XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis ADP-ribosylation factor-like protein 8A (arl8a) CSB-YP754212XBF
CSB-EP754212XBF
CSB-BP754212XBF
CSB-MP754212XBF
CSB-EP754212XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse PEST proteolytic signal-containing nuclear protein (Pcnp) CSB-YP754213MO
CSB-EP754213MO
CSB-BP754213MO
CSB-MP754213MO
CSB-EP754213MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Pancreatic triacylglycerol lipase (Pnlip) CSB-YP754214MO
CSB-BP754214MO
CSB-MP754214MO
CSB-EP754214MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Spermatogenesis-associated protein 24 (Spata24) CSB-YP754215MO
CSB-EP754215MO
CSB-BP754215MO
CSB-MP754215MO
CSB-EP754215MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Hydroxyacylglutathione hydrolase, mitochondrial (hagh), partial CSB-YP754216DIL
CSB-EP754216DIL
CSB-BP754216DIL
CSB-MP754216DIL
CSB-EP754216DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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