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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Geobacter sulfurreducens Membrane protein insertase YidC (yidC), partial CSB-YP754999GBK1
CSB-EP754999GBK1
CSB-BP754999GBK1
CSB-MP754999GBK1
CSB-EP754999GBK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP755000GBK
CSB-EP755000GBK
CSB-BP755000GBK
CSB-MP755000GBK
CSB-EP755000GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Porphobilinogen deaminase (hemC) CSB-YP755001GBK
CSB-EP755001GBK
CSB-BP755001GBK
CSB-MP755001GBK
CSB-EP755001GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP755002GBK
CSB-EP755002GBK
CSB-BP755002GBK
CSB-MP755002GBK
CSB-EP755002GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Isoleucine--tRNA ligase (ileS), partial CSB-YP755003GBK
CSB-EP755003GBK
CSB-BP755003GBK
CSB-MP755003GBK
CSB-EP755003GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 30S ribosomal protein S21 2 (rpsU2) CSB-YP755004GBK
CSB-EP755004GBK
CSB-BP755004GBK
CSB-MP755004GBK
CSB-EP755004GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP755005GBK
CSB-EP755005GBK
CSB-BP755005GBK
CSB-MP755005GBK
CSB-EP755005GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP755006GBK
CSB-EP755006GBK
CSB-BP755006GBK
CSB-MP755006GBK
CSB-EP755006GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 50S ribosomal protein L1 (rplA) CSB-YP755007GBK
CSB-EP755007GBK
CSB-BP755007GBK
CSB-MP755007GBK
CSB-EP755007GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 50S ribosomal protein L22 (rplV) CSB-YP755008GBK
CSB-EP755008GBK
CSB-BP755008GBK
CSB-MP755008GBK
CSB-EP755008GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 30S ribosomal protein S14 type Z (rpsZ) CSB-YP755009GBK
CSB-EP755009GBK
CSB-BP755009GBK
CSB-MP755009GBK
CSB-EP755009GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 50S ribosomal protein L15 (rplO) CSB-YP755010GBK
CSB-EP755010GBK
CSB-BP755010GBK
CSB-MP755010GBK
CSB-EP755010GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 50S ribosomal protein L17 (rplQ) CSB-YP755011GBK
CSB-EP755011GBK
CSB-BP755011GBK
CSB-MP755011GBK
CSB-EP755011GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 8-amino-7-oxononanoate synthase (bioF) CSB-YP755012GBK
CSB-EP755012GBK
CSB-BP755012GBK
CSB-MP755012GBK
CSB-EP755012GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Maf-like protein GSU2545 (GSU2545) CSB-YP755013GBK
CSB-EP755013GBK
CSB-BP755013GBK
CSB-MP755013GBK
CSB-EP755013GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Anthranilate phosphoribosyltransferase (trpD) CSB-YP755014GBK
CSB-EP755014GBK
CSB-BP755014GBK
CSB-MP755014GBK
CSB-EP755014GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Lipoprotein-releasing system ATP-binding protein LolD (lolD) CSB-YP755015GBK
CSB-EP755015GBK
CSB-BP755015GBK
CSB-MP755015GBK
CSB-EP755015GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Thymidylate kinase (tmk) CSB-YP755016GBK
CSB-EP755016GBK
CSB-BP755016GBK
CSB-MP755016GBK
CSB-EP755016GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Phosphoheptose isomerase (gmhA) CSB-YP755017GBK
CSB-EP755017GBK
CSB-BP755017GBK
CSB-MP755017GBK
CSB-EP755017GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 3-dehydroquinate synthase (aroB) CSB-YP755018GBK
CSB-EP755018GBK
CSB-BP755018GBK
CSB-MP755018GBK
CSB-EP755018GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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