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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Chaperone protein DnaJ 1 (dnaJ1) CSB-YP754979MLA
CSB-EP754979MLA
CSB-BP754979MLA
CSB-MP754979MLA
CSB-EP754979MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloalkane dehalogenase (dhmA) CSB-YP754980MLA
CSB-EP754980MLA
CSB-BP754980MLA
CSB-MP754980MLA
CSB-EP754980MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cobalamin synthase (cobS), partial CSB-YP754981MLA1
CSB-EP754981MLA1
CSB-BP754981MLA1
CSB-MP754981MLA1
CSB-EP754981MLA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP754982MLA
CSB-EP754982MLA
CSB-BP754982MLA
CSB-MP754982MLA
CSB-EP754982MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pup--protein ligase (pafA) CSB-YP754983MLA
CSB-EP754983MLA
CSB-BP754983MLA
CSB-MP754983MLA
CSB-EP754983MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable phosphoketolase (MAP_1573c), partial CSB-YP754984MLA
CSB-EP754984MLA
CSB-BP754984MLA
CSB-MP754984MLA
CSB-EP754984MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine cleavage system H protein (gcvH) CSB-YP754985MLA
CSB-EP754985MLA
CSB-BP754985MLA
CSB-MP754985MLA
CSB-EP754985MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L35 (rpmI) CSB-YP754986MLA
CSB-EP754986MLA
CSB-BP754986MLA
CSB-MP754986MLA
CSB-EP754986MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Group 1 truncated hemoglobin glbN (glbN) CSB-YP754987MLA
CSB-EP754987MLA
CSB-BP754987MLA
CSB-MP754987MLA
CSB-EP754987MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Protein Vpr (vpr) CSB-YP754988HDAG
CSB-EP754988HDAG
CSB-BP754988HDAG
CSB-MP754988HDAG
CSB-EP754988HDAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transaldolase (tal) CSB-YP754989MLA
CSB-EP754989MLA
CSB-BP754989MLA
CSB-MP754989MLA
CSB-EP754989MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carbamoyl-phosphate synthase small chain (carA) CSB-YP754990MLA
CSB-EP754990MLA
CSB-BP754990MLA
CSB-MP754990MLA
CSB-EP754990MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shikimate kinase (aroK) CSB-YP754991MLA
CSB-EP754991MLA
CSB-BP754991MLA
CSB-MP754991MLA
CSB-EP754991MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potassium-transporting ATPase A chain (kdpA), partial CSB-YP754992MLA
CSB-EP754992MLA
CSB-BP754992MLA
CSB-MP754992MLA
CSB-EP754992MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype B Protein Rev (rev) CSB-YP754993HME
CSB-EP754993HME
CSB-BP754993HME
CSB-MP754993HME
CSB-EP754993HME-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alpha-keto-acid decarboxylase (kdc), partial CSB-YP754994MLA
CSB-EP754994MLA
CSB-BP754994MLA
CSB-MP754994MLA
CSB-EP754994MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP754995MLA
CSB-EP754995MLA
CSB-BP754995MLA
CSB-MP754995MLA
CSB-EP754995MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP754996MLA
CSB-EP754996MLA
CSB-BP754996MLA
CSB-MP754996MLA
CSB-EP754996MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0133 protein MAP_0317c (MAP_0317c) CSB-YP754997MLA
CSB-EP754997MLA
CSB-BP754997MLA
CSB-MP754997MLA
CSB-EP754997MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NADH-quinone oxidoreductase subunit I 1 (nuoI1) CSB-YP754998MLA
CSB-EP754998MLA
CSB-BP754998MLA
CSB-MP754998MLA
CSB-EP754998MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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