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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhodopseudomonas palustris UPF0391 membrane protein RPA3029 (RPA3029), partial CSB-YP754037RLU
CSB-EP754037RLU
CSB-BP754037RLU
CSB-MP754037RLU
CSB-EP754037RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP754038RLU
CSB-EP754038RLU
CSB-BP754038RLU
CSB-MP754038RLU
CSB-EP754038RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris NADH-quinone oxidoreductase subunit K 1 (nuoK1), partial CSB-YP754039RLU1
CSB-EP754039RLU1
CSB-BP754039RLU1
CSB-MP754039RLU1
CSB-EP754039RLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Lipid-A-disaccharide synthase (lpxB) CSB-YP754040RLU
CSB-EP754040RLU
CSB-BP754040RLU
CSB-MP754040RLU
CSB-EP754040RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Arginine--tRNA ligase (argS), partial CSB-YP754041RLU
CSB-EP754041RLU
CSB-BP754041RLU
CSB-MP754041RLU
CSB-EP754041RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris N utilization substance protein B homolog (nusB) CSB-YP754042RLU
CSB-EP754042RLU
CSB-BP754042RLU
CSB-MP754042RLU
CSB-EP754042RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Large-conductance mechanosensitive channel (mscL), partial CSB-YP754043RLU1
CSB-EP754043RLU1
CSB-BP754043RLU1
CSB-MP754043RLU1
CSB-EP754043RLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Alkanesulfonate monooxygenase (ssuD) CSB-YP754044RLU
CSB-EP754044RLU
CSB-BP754044RLU
CSB-MP754044RLU
CSB-EP754044RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Valine--tRNA ligase (valS), partial CSB-YP754045RLU
CSB-EP754045RLU
CSB-BP754045RLU
CSB-MP754045RLU
CSB-EP754045RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP754046RLU
CSB-EP754046RLU
CSB-BP754046RLU
CSB-MP754046RLU
CSB-EP754046RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Ketol-acid reductoisomerase (ilvC) CSB-YP754047RLU
CSB-EP754047RLU
CSB-BP754047RLU
CSB-MP754047RLU
CSB-EP754047RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Probable potassium transport system protein kup 3 (kup3), partial CSB-YP754048RLU
CSB-EP754048RLU
CSB-BP754048RLU
CSB-MP754048RLU
CSB-EP754048RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Coenzyme PQQ synthesis protein B (pqqB) CSB-YP754049RLU
CSB-EP754049RLU
CSB-BP754049RLU
CSB-MP754049RLU
CSB-EP754049RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 30S ribosomal protein S4 (rpsD) CSB-YP754050RLU
CSB-EP754050RLU
CSB-BP754050RLU
CSB-MP754050RLU
CSB-EP754050RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein (bchL) CSB-YP754051RLU
CSB-EP754051RLU
CSB-BP754051RLU
CSB-MP754051RLU
CSB-EP754051RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Chorismate synthase (aroC) CSB-YP754052RLU
CSB-EP754052RLU
CSB-BP754052RLU
CSB-MP754052RLU
CSB-EP754052RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP754053RLU
CSB-EP754053RLU
CSB-BP754053RLU
CSB-MP754053RLU
CSB-EP754053RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Probable multidrug resistance protein norM (norM), partial CSB-YP754054RLU
CSB-EP754054RLU
CSB-BP754054RLU
CSB-MP754054RLU
CSB-EP754054RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Phosphonates import ATP-binding protein PhnC 1 (phnC1) CSB-YP754055RLU
CSB-EP754055RLU
CSB-BP754055RLU
CSB-MP754055RLU
CSB-EP754055RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Peptide deformylase (def) CSB-YP754056RLU
CSB-EP754056RLU
CSB-BP754056RLU
CSB-MP754056RLU
CSB-EP754056RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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