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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Danio rerio Synaptosomal-associated protein 25-B (snap25b) CSB-YP754237DIL
CSB-EP754237DIL
CSB-BP754237DIL
CSB-MP754237DIL
CSB-EP754237DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio E3 ubiquitin-protein ligase RNF185 (rnf185), partial CSB-YP754238DIL1
CSB-EP754238DIL1
CSB-BP754238DIL1
CSB-MP754238DIL1
CSB-EP754238DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Embigin (EMB), partial CSB-YP754239HU
CSB-EP754239HU
CSB-BP754239HU
CSB-MP754239HU
CSB-EP754239HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Putative sodium-coupled neutral amino acid transporter 10 (slc38a10), partial CSB-YP754240XBE
CSB-EP754240XBE
CSB-BP754240XBE
CSB-MP754240XBE
CSB-EP754240XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein arginine N-methyltransferase 7 (prmt7), partial CSB-YP754241XBE
CSB-EP754241XBE
CSB-BP754241XBE
CSB-MP754241XBE
CSB-EP754241XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human F-box/LRR-repeat protein 19 (FBXL19) CSB-YP754242HU
CSB-EP754242HU
CSB-BP754242HU
CSB-MP754242HU
CSB-EP754242HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Repulsive guidance molecule A (Rgma) CSB-YP754243MO
CSB-EP754243MO
CSB-BP754243MO
CSB-MP754243MO
CSB-EP754243MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse GPI transamidase component PIG-S (Pigs), partial CSB-YP754244MO1
CSB-EP754244MO1
CSB-BP754244MO1
CSB-MP754244MO1
CSB-EP754244MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Sperm-associated antigen 11A (SPAG11A) CSB-YP754245HU
CSB-EP754245HU
CSB-BP754245HU
CSB-MP754245HU
CSB-EP754245HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse UDP-glucuronosyltransferase 2A2 (Ugt2a2), partial CSB-YP754246MO
CSB-EP754246MO
CSB-BP754246MO
CSB-MP754246MO
CSB-EP754246MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Vacuolar fusion protein MON1 homolog A (Mon1a) CSB-YP754247MO
CSB-EP754247MO
CSB-BP754247MO
CSB-MP754247MO
CSB-EP754247MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Proteasome-associated protein ECM29 homolog (Ecm29), partial CSB-YP754248MO
CSB-EP754248MO
CSB-BP754248MO
CSB-MP754248MO
CSB-EP754248MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Chromodomain-helicase-DNA-binding protein 4 (Chd4), partial CSB-YP754249MO
CSB-EP754249MO
CSB-BP754249MO
CSB-MP754249MO
CSB-EP754249MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse 2-aminoethanethiol dioxygenase (Ado) CSB-YP754250MO
CSB-EP754250MO
CSB-BP754250MO
CSB-MP754250MO
CSB-EP754250MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse UPF0704 protein C6orf165 homolog CSB-YP754251MO
CSB-EP754251MO
CSB-BP754251MO
CSB-MP754251MO
CSB-EP754251MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ribonucleoside-diphosphate reductase subunit M2 B (Rrm2b) CSB-YP754252MO
CSB-EP754252MO
CSB-BP754252MO
CSB-MP754252MO
CSB-EP754252MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio CTP synthase 1 (ctps), partial CSB-YP754253DIL
CSB-EP754253DIL
CSB-BP754253DIL
CSB-MP754253DIL
CSB-EP754253DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Inactive serine protease 54 (PRSS54) CSB-YP754254HU
CSB-EP754254HU
CSB-BP754254HU
CSB-MP754254HU
CSB-EP754254HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tubulin alpha-3E chain (TUBA3E) CSB-YP754255HU
CSB-EP754255HU
CSB-BP754255HU
CSB-MP754255HU
CSB-EP754255HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Kelch-like protein 35 (KLHL35) CSB-YP754256HU
CSB-EP754256HU
CSB-BP754256HU
CSB-MP754256HU
CSB-EP754256HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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