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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human Mediator of RNA polymerase II transcription subunit 13-like (MED13L), partial CSB-YP754639HU
CSB-EP754639HU
CSB-BP754639HU
CSB-MP754639HU
CSB-EP754639HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyaena hyaena Cytochrome b (MT-CYB), partial CSB-YP754640HDAO
CSB-EP754640HDAO
CSB-BP754640HDAO
CSB-MP754640HDAO
CSB-EP754640HDAO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Civettictis civetta Cytochrome b (MT-CYB), partial CSB-YP754641CHAZ
CSB-EP754641CHAZ
CSB-BP754641CHAZ
CSB-MP754641CHAZ
CSB-EP754641CHAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsormidium bilatum Protein psbN (psbN), partial CSB-YP754642KAK
CSB-EP754642KAK
CSB-BP754642KAK
CSB-MP754642KAK
CSB-EP754642KAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome b6 (petB), partial CSB-YP754643CNB1
CSB-EP754643CNB1
CSB-BP754643CNB1
CSB-MP754643CNB1
CSB-EP754643CNB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bowenia serrulata 30S ribosomal protein S7, chloroplastic (rps7) CSB-YP754644BDAM
CSB-EP754644BDAM
CSB-BP754644BDAM
CSB-MP754644BDAM
CSB-EP754644BDAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cedrus deodara Photosystem II reaction center protein L (psbL), partial CSB-YP754645CFM
CSB-EP754645CFM
CSB-BP754645CFM
CSB-MP754645CFM
CSB-EP754645CFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cycas revoluta Photosystem II reaction center protein T (psbT), partial CSB-YP754646EPY
CSB-EP754646EPY
CSB-BP754646EPY
CSB-MP754646EPY
CSB-EP754646EPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chimaphila umbellata Maturase K (matK), partial CSB-YP754647CIAA
CSB-EP754647CIAA
CSB-BP754647CIAA
CSB-MP754647CIAA
CSB-EP754647CIAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hylocomium splendens Protein psbN (psbN), partial CSB-YP754648HRI
CSB-EP754648HRI
CSB-BP754648HRI
CSB-MP754648HRI
CSB-EP754648HRI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viridovipera stejnegeri Snake venom serine protease KN13 CSB-YP754649VDN
CSB-EP754649VDN
CSB-BP754649VDN
CSB-MP754649VDN
CSB-EP754649VDN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viridovipera stejnegeri Snake venom serine protease KN11 CSB-YP754650VDN
CSB-EP754650VDN
CSB-BP754650VDN
CSB-MP754650VDN
CSB-EP754650VDN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative uncharacterized protein FP248 (FP248) CSB-YP754651HU
CSB-EP754651HU
CSB-BP754651HU
CSB-MP754651HU
CSB-EP754651HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein FAM57B (FAM57B), partial CSB-YP754652HU1
CSB-EP754652HU1
CSB-BP754652HU1
CSB-MP754652HU1
CSB-EP754652HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viridovipera stejnegeri Coagulation factor IX/factor X-binding protein subunit B2 CSB-YP754653VDN
CSB-EP754653VDN
CSB-BP754653VDN
CSB-MP754653VDN
CSB-EP754653VDN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Sodium/potassium/calcium exchanger 5 (SLC24A5), partial CSB-YP754654HU
CSB-EP754654HU
CSB-BP754654HU
CSB-MP754654HU
CSB-EP754654HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Class E basic helix-loop-helix protein 22 (BHLHE22) CSB-YP754655CH
CSB-EP754655CH
CSB-BP754655CH
CSB-MP754655CH
CSB-EP754655CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tubulin alpha-1A chain (TUBA1A) CSB-YP754656HU
CSB-EP754656HU
CSB-BP754656HU
CSB-MP754656HU
CSB-EP754656HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sminthopsis youngsoni Sperm protamine P1 (PRM1) CSB-YP754657SGAY
CSB-EP754657SGAY
CSB-BP754657SGAY
CSB-MP754657SGAY
CSB-EP754657SGAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sminthopsis archeri Sperm protamine P1 (PRM1) CSB-YP754658SGAZ
CSB-EP754658SGAZ
CSB-BP754658SGAZ
CSB-MP754658SGAZ
CSB-EP754658SGAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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