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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Treponema denticola Methionine--tRNA ligase (metG), partial CSB-YP754939TPP
CSB-EP754939TPP
CSB-BP754939TPP
CSB-MP754939TPP
CSB-EP754939TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola 50S ribosomal protein L32 (rpmF) CSB-YP754940TPP
CSB-EP754940TPP
CSB-BP754940TPP
CSB-MP754940TPP
CSB-EP754940TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola Ornithine carbamoyltransferase, catabolic (arcB) CSB-YP754941TPP
CSB-EP754941TPP
CSB-BP754941TPP
CSB-MP754941TPP
CSB-EP754941TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP754942TPP
CSB-EP754942TPP
CSB-BP754942TPP
CSB-MP754942TPP
CSB-EP754942TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola 50S ribosomal protein L15 (rplO) CSB-YP754943TPP
CSB-EP754943TPP
CSB-BP754943TPP
CSB-MP754943TPP
CSB-EP754943TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola 50S ribosomal protein L5 (rplE) CSB-YP754944TPP
CSB-EP754944TPP
CSB-BP754944TPP
CSB-MP754944TPP
CSB-EP754944TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola 50S ribosomal protein L22 (rplV) CSB-YP754945TPP
CSB-EP754945TPP
CSB-BP754945TPP
CSB-MP754945TPP
CSB-EP754945TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola Glycine/sarcosine/betaine reductase complex component A (grdA) CSB-YP754946TPP
CSB-EP754946TPP
CSB-BP754946TPP
CSB-MP754946TPP
CSB-EP754946TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP754947TPP
CSB-EP754947TPP
CSB-BP754947TPP
CSB-MP754947TPP
CSB-EP754947TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola Glucosamine-6-phosphate deaminase (nagB) CSB-YP754948TPP
CSB-EP754948TPP
CSB-BP754948TPP
CSB-MP754948TPP
CSB-EP754948TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP754949TPP
CSB-EP754949TPP
CSB-BP754949TPP
CSB-MP754949TPP
CSB-EP754949TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP754950MLA
CSB-EP754950MLA
CSB-BP754950MLA
CSB-MP754950MLA
CSB-EP754950MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L13 (rplM) CSB-YP754951MLA
CSB-EP754951MLA
CSB-BP754951MLA
CSB-MP754951MLA
CSB-EP754951MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adenylate kinase (adk) CSB-YP754952MLA
CSB-EP754952MLA
CSB-BP754952MLA
CSB-MP754952MLA
CSB-EP754952MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L18 (rplR) CSB-YP754953MLA
CSB-EP754953MLA
CSB-BP754953MLA
CSB-MP754953MLA
CSB-EP754953MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L16 (rplP) CSB-YP754954MLA
CSB-EP754954MLA
CSB-BP754954MLA
CSB-MP754954MLA
CSB-EP754954MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized oxidoreductase MAP_4149 (MAP_4149) CSB-YP754955MLA
CSB-EP754955MLA
CSB-BP754955MLA
CSB-MP754955MLA
CSB-EP754955MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L7/L12 (rplL) CSB-YP754956MLA
CSB-EP754956MLA
CSB-BP754956MLA
CSB-MP754956MLA
CSB-EP754956MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative S-adenosyl-L-methionine-dependent methyltransferase MAP_4064c (MAP_4064c) CSB-YP754957MLA
CSB-EP754957MLA
CSB-BP754957MLA
CSB-MP754957MLA
CSB-EP754957MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0336 protein MAP_3996c (MAP_3996c) CSB-YP754958MLA
CSB-EP754958MLA
CSB-BP754958MLA
CSB-MP754958MLA
CSB-EP754958MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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