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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Protein fmtA (fmtA) CSB-YP756905SKV
CSB-EP756905SKV
CSB-BP756905SKV
CSB-MP756905SKV
CSB-EP756905SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Peptide chain release factor 3 (prfC), partial CSB-YP756906SKV
CSB-EP756906SKV
CSB-BP756906SKV
CSB-MP756906SKV
CSB-EP756906SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0738 protein SAR0972 (SAR0972) CSB-YP756907SKV
CSB-EP756907SKV
CSB-BP756907SKV
CSB-MP756907SKV
CSB-EP756907SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP756908SKV
CSB-EP756908SKV
CSB-BP756908SKV
CSB-MP756908SKV
CSB-EP756908SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ornithine aminotransferase 2 (rocD2) CSB-YP756909SKV
CSB-EP756909SKV
CSB-BP756909SKV
CSB-MP756909SKV
CSB-EP756909SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Na (+)/H (+) antiporter subunit C1 (mnhC1), partial CSB-YP756910SKV1
CSB-EP756910SKV1
CSB-BP756910SKV1
CSB-MP756910SKV1
CSB-EP756910SKV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Organic hydroperoxide resistance protein-like (SAR0859) CSB-YP756911SKV
CSB-EP756911SKV
CSB-BP756911SKV
CSB-MP756911SKV
CSB-EP756911SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0042 nucleotide-binding protein SAR0820 (SAR0820) CSB-YP756912SKV
CSB-EP756912SKV
CSB-BP756912SKV
CSB-MP756912SKV
CSB-EP756912SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus HPr kinase/phosphorylase (hprK) CSB-YP756913SKV
CSB-EP756913SKV
CSB-BP756913SKV
CSB-MP756913SKV
CSB-EP756913SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized protein SAR0806 (SAR0806) CSB-YP756914SKV
CSB-EP756914SKV
CSB-BP756914SKV
CSB-MP756914SKV
CSB-EP756914SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Lipoteichoic acid synthase (ltaS), partial CSB-YP756915SKV1
CSB-EP756915SKV1
CSB-BP756915SKV1
CSB-MP756915SKV1
CSB-EP756915SKV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable copper-transporting P-type ATPase B (copB), partial CSB-YP756916SKV
CSB-EP756916SKV
CSB-BP756916SKV
CSB-MP756916SKV
CSB-EP756916SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0741 protein SAR0599 (SAR0599) CSB-YP756917SKV
CSB-EP756917SKV
CSB-BP756917SKV
CSB-MP756917SKV
CSB-EP756917SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine-aspartate repeat-containing protein C (sdrC), partial CSB-YP756918SKV
CSB-EP756918SKV
CSB-BP756918SKV
CSB-MP756918SKV
CSB-EP756918SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP756919SKV
CSB-EP756919SKV
CSB-BP756919SKV
CSB-MP756919SKV
CSB-EP756919SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine acetyltransferase (cysE) CSB-YP756920SKV
CSB-EP756920SKV
CSB-BP756920SKV
CSB-MP756920SKV
CSB-EP756920SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus tRNA (Ile)-lysidine synthase (tilS) CSB-YP756921SKV
CSB-EP756921SKV
CSB-BP756921SKV
CSB-MP756921SKV
CSB-EP756921SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative septation protein spoVG (spoVG) CSB-YP756922SKV
CSB-EP756922SKV
CSB-BP756922SKV
CSB-MP756922SKV
CSB-EP756922SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Recombination protein RecR (recR) CSB-YP756923SKV
CSB-EP756923SKV
CSB-BP756923SKV
CSB-MP756923SKV
CSB-EP756923SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized lipoprotein SAR0442 (SAR0442) CSB-YP756924SKV
CSB-EP756924SKV
CSB-BP756924SKV
CSB-MP756924SKV
CSB-EP756924SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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