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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP756503BIK
CSB-EP756503BIK
CSB-BP756503BIK
CSB-MP756503BIK
CSB-EP756503BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide chain release factor 3 (prfC), partial CSB-YP756504BIK
CSB-EP756504BIK
CSB-BP756504BIK
CSB-MP756504BIK
CSB-EP756504BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type IV secretion system protein ptlC homolog (ptlC), partial CSB-YP756505BIK
CSB-EP756505BIK
CSB-BP756505BIK
CSB-MP756505BIK
CSB-EP756505BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP756506BIK
CSB-EP756506BIK
CSB-BP756506BIK
CSB-MP756506BIK
CSB-EP756506BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP756507BIK
CSB-EP756507BIK
CSB-BP756507BIK
CSB-MP756507BIK
CSB-EP756507BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit b (atpF), partial CSB-YP756508BIK
CSB-EP756508BIK
CSB-BP756508BIK
CSB-MP756508BIK
CSB-EP756508BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homoserine O-acetyltransferase (metX) CSB-YP756509BIK
CSB-EP756509BIK
CSB-BP756509BIK
CSB-MP756509BIK
CSB-EP756509BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0102 protein BPP4042 (BPP4042) CSB-YP756510BIK
CSB-EP756510BIK
CSB-BP756510BIK
CSB-MP756510BIK
CSB-EP756510BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycerol kinase (glpK), partial CSB-YP756511BIK
CSB-EP756511BIK
CSB-BP756511BIK
CSB-MP756511BIK
CSB-EP756511BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-dehydroquinate dehydratase (aroQ) CSB-YP756512BIK
CSB-EP756512BIK
CSB-BP756512BIK
CSB-MP756512BIK
CSB-EP756512BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative iron-sulfur cluster insertion protein ErpA (erpA) CSB-YP756513BIK
CSB-EP756513BIK
CSB-BP756513BIK
CSB-MP756513BIK
CSB-EP756513BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Urease accessory protein UreF (ureF) CSB-YP756514BIK
CSB-EP756514BIK
CSB-BP756514BIK
CSB-MP756514BIK
CSB-EP756514BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP756515BIK
CSB-EP756515BIK
CSB-BP756515BIK
CSB-MP756515BIK
CSB-EP756515BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipid A palmitoyltransferase PagP (pagP) CSB-YP756516BIK
CSB-EP756516BIK
CSB-BP756516BIK
CSB-MP756516BIK
CSB-EP756516BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide deformylase 2 (def2) CSB-YP756517BIK
CSB-EP756517BIK
CSB-BP756517BIK
CSB-MP756517BIK
CSB-EP756517BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP756518BIK
CSB-EP756518BIK
CSB-BP756518BIK
CSB-MP756518BIK
CSB-EP756518BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP756519BIK
CSB-EP756519BIK
CSB-BP756519BIK
CSB-MP756519BIK
CSB-EP756519BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serine--tRNA ligase (serS) CSB-YP756520BIK
CSB-EP756520BIK
CSB-BP756520BIK
CSB-MP756520BIK
CSB-EP756520BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP756521BIK
CSB-EP756521BIK
CSB-BP756521BIK
CSB-MP756521BIK
CSB-EP756521BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable parvulin-type peptidyl-prolyl cis-trans isomerase (BPP3352) CSB-YP756522BIK
CSB-EP756522BIK
CSB-BP756522BIK
CSB-MP756522BIK
CSB-EP756522BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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