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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Xenopus laevis Methyltransferase-like protein 13 (mettl13), partial CSB-YP757505XBE
CSB-EP757505XBE
CSB-BP757505XBE
CSB-MP757505XBE
CSB-EP757505XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Mediator of RNA polymerase II transcription subunit 20 (med20) CSB-YP757506XBE
CSB-EP757506XBE
CSB-BP757506XBE
CSB-MP757506XBE
CSB-EP757506XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis NEDD8-activating enzyme E1 regulatory subunit (nae1), partial CSB-YP757507XBE
CSB-EP757507XBE
CSB-BP757507XBE
CSB-MP757507XBE
CSB-EP757507XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Leucine-rich repeat-containing protein 8E (lrrc8e), partial CSB-YP757508XBE
CSB-EP757508XBE
CSB-BP757508XBE
CSB-MP757508XBE
CSB-EP757508XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Eukaryotic translation initiation factor 4E type 3-B (eif4e3-b) CSB-YP757509XBE
CSB-EP757509XBE
CSB-BP757509XBE
CSB-MP757509XBE
CSB-EP757509XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis LSM domain-containing protein 1-B (lsmd1-b) CSB-YP757510XBE
CSB-EP757510XBE
CSB-BP757510XBE
CSB-MP757510XBE
CSB-EP757510XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Nucleotide exchange factor SIL1 (sil1) CSB-YP757511XBE
CSB-EP757511XBE
CSB-BP757511XBE
CSB-MP757511XBE
CSB-EP757511XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis 60S ribosomal protein L31 (rpl31) CSB-YP757512XBE
CSB-EP757512XBE
CSB-BP757512XBE
CSB-MP757512XBE
CSB-EP757512XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Serine/threonine-protein phosphatase 4 regulatory subunit 4 (PPP4R4), partial CSB-YP757513HU
CSB-EP757513HU
CSB-BP757513HU
CSB-MP757513HU
CSB-EP757513HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein dpy-19 homolog 2 (DPY19L2), partial CSB-YP757514HU
CSB-EP757514HU
CSB-BP757514HU
CSB-MP757514HU
CSB-EP757514HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio FGGY carbohydrate kinase domain-containing protein (fggy), partial CSB-YP757515DIL
CSB-EP757515DIL
CSB-BP757515DIL
CSB-MP757515DIL
CSB-EP757515DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein tweety homolog 2-like (ttyh2l), partial CSB-YP757516DIL1
CSB-EP757516DIL1
CSB-BP757516DIL1
CSB-MP757516DIL1
CSB-EP757516DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio WD repeat-containing protein 82 (wdr82) CSB-YP757517DIL
CSB-EP757517DIL
CSB-BP757517DIL
CSB-MP757517DIL
CSB-EP757517DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein C2orf55 (C2orf55), partial CSB-YP757518HU
CSB-EP757518HU
CSB-BP757518HU
CSB-MP757518HU
CSB-EP757518HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse U2 snRNP-associated SURP motif-containing protein (U2surp), partial CSB-YP757519MO
CSB-EP757519MO
CSB-BP757519MO
CSB-MP757519MO
CSB-EP757519MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis LIM domain-binding protein 1 (ldb1) CSB-YP757520XBF
CSB-EP757520XBF
CSB-BP757520XBF
CSB-MP757520XBF
CSB-EP757520XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Protein LSM14 homolog A (lsm14a) CSB-YP757521XBF
CSB-EP757521XBF
CSB-BP757521XBF
CSB-MP757521XBF
CSB-EP757521XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Mitochondrial import inner membrane translocase subunit TIM50 (timm50), partial CSB-YP757522DIL1
CSB-EP757522DIL1
CSB-BP757522DIL1
CSB-MP757522DIL1
CSB-EP757522DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio B-cell CLL/lymphoma 7 protein family member B-A (bcl7ba) CSB-YP757523DIL
CSB-EP757523DIL
CSB-BP757523DIL
CSB-MP757523DIL
CSB-EP757523DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Cell differentiation protein RCD1 homolog (rqcd1) CSB-YP757524DIL
CSB-EP757524DIL
CSB-BP757524DIL
CSB-MP757524DIL
CSB-EP757524DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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