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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human Protein FAM180B (FAM180B), partial CSB-YP757545HU1
CSB-EP757545HU1
CSB-BP757545HU1
CSB-MP757545HU1
CSB-EP757545HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio MAGUK p55 subfamily member 7 (mpp7), partial CSB-YP757546DIL
CSB-EP757546DIL
CSB-BP757546DIL
CSB-MP757546DIL
CSB-EP757546DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Shootin-1, partial CSB-YP757547DIL
CSB-EP757547DIL
CSB-BP757547DIL
CSB-MP757547DIL
CSB-EP757547DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Nucleoporin-like protein 2 (nupl2) CSB-YP757548DIL
CSB-EP757548DIL
CSB-BP757548DIL
CSB-MP757548DIL
CSB-EP757548DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative ATP-dependent RNA helicase DHX57 (DHX57), partial CSB-YP757549HU
CSB-EP757549HU
CSB-BP757549HU
CSB-MP757549HU
CSB-EP757549HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse EF-hand calcium-binding domain-containing protein 6 (Efcab6), partial CSB-YP757550MO
CSB-EP757550MO
CSB-BP757550MO
CSB-MP757550MO
CSB-EP757550MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Lysine-specific demethylase 2B (Kdm2b), partial CSB-YP757551MO
CSB-EP757551MO
CSB-BP757551MO
CSB-MP757551MO
CSB-EP757551MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Glycoprotein endo-alpha-1,2-mannosidase-like protein (Maneal), partial CSB-YP757552MO
CSB-EP757552MO
CSB-BP757552MO
CSB-MP757552MO
CSB-EP757552MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human General transcription factor IIH subunit 2-like protein (GTF2H2C) CSB-YP757553HU
CSB-EP757553HU
CSB-BP757553HU
CSB-MP757553HU
CSB-EP757553HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human LETM1 domain-containing protein 1 (LETMD1), partial CSB-YP757554HU
CSB-EP757554HU
CSB-BP757554HU
CSB-MP757554HU
CSB-EP757554HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Protein tweety homolog 2 (ttyh2), partial CSB-YP757555XBF1
CSB-EP757555XBF1
CSB-BP757555XBF1
CSB-MP757555XBF1
CSB-EP757555XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Zygotic DNA replication licensing factor mcm6 (zmcm6), partial CSB-YP757556XBF
CSB-EP757556XBF
CSB-BP757556XBF
CSB-MP757556XBF
CSB-EP757556XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Phosducin-like protein 3 (pdcl3) CSB-YP757557DIL
CSB-EP757557DIL
CSB-BP757557DIL
CSB-MP757557DIL
CSB-EP757557DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Transportin-3 (Tnpo3), partial CSB-YP757558MO
CSB-EP757558MO
CSB-BP757558MO
CSB-MP757558MO
CSB-EP757558MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Centrosomal protein of 85 kDa (CEP85) CSB-YP757559HU
CSB-EP757559HU
CSB-BP757559HU
CSB-MP757559HU
CSB-EP757559HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tetratricopeptide repeat protein GNN (GNN), partial CSB-YP757560HU
CSB-EP757560HU
CSB-BP757560HU
CSB-MP757560HU
CSB-EP757560HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Ragulator complex protein LAMTOR1 (lamtor1) CSB-YP757561XBF
CSB-EP757561XBF
CSB-BP757561XBF
CSB-MP757561XBF
CSB-EP757561XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis UPF0608 protein C19orf42 homolog (TEgg109m01.1), partial CSB-YP757562XBF
CSB-EP757562XBF
CSB-BP757562XBF
CSB-MP757562XBF
CSB-EP757562XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Serine protease ami (ami) CSB-YP757563XBF
CSB-EP757563XBF
CSB-BP757563XBF
CSB-MP757563XBF
CSB-EP757563XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Eukaryotic translation initiation factor 3 subunit H (eif3h) CSB-YP757564XBF
CSB-EP757564XBF
CSB-BP757564XBF
CSB-MP757564XBF
CSB-EP757564XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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