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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Gossypium hirsutum Tubulin alpha-4 chain CSB-YP757806GHB
CSB-EP757806GHB
CSB-BP757806GHB
CSB-MP757806GHB
CSB-EP757806GHB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica MADS-box transcription factor 23 (MADS23) CSB-YP757807OFG
CSB-EP757807OFG
CSB-BP757807OFG
CSB-MP757807OFG
CSB-EP757807OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Heat stress transcription factor C-1a (HSFC1A) CSB-YP757808OFG
CSB-EP757808OFG
CSB-BP757808OFG
CSB-MP757808OFG
CSB-EP757808OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio RNA-binding protein PNO1 (pno1) CSB-YP757809DIL
CSB-EP757809DIL
CSB-BP757809DIL
CSB-MP757809DIL
CSB-EP757809DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ran-binding protein 10 (Ranbp10) CSB-YP757810MO
CSB-EP757810MO
CSB-BP757810MO
CSB-MP757810MO
CSB-EP757810MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorella protothecoides Light-independent protochlorophyllide reductase subunit B (chlB), partial CSB-YP757811CJG
CSB-EP757811CJG
CSB-BP757811CJG
CSB-MP757811CJG
CSB-EP757811CJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyprinus carpio Radial spoke head 1 homolog (rsph1) CSB-YP757812EQE
CSB-EP757812EQE
CSB-BP757812EQE
CSB-MP757812EQE
CSB-EP757812EQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Transcription factor AP-2-epsilon (Tfap2e) CSB-YP757813MO
CSB-EP757813MO
CSB-BP757813MO
CSB-MP757813MO
CSB-EP757813MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Eukaryotic translation initiation factor 1A (Eif1a) CSB-YP757814RA
CSB-EP757814RA
CSB-BP757814RA
CSB-MP757814RA
CSB-EP757814RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Dead end protein homolog 1 (Dnd1) CSB-YP757815MO
CSB-EP757815MO
CSB-BP757815MO
CSB-MP757815MO
CSB-EP757815MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Desmoglein-4 (Dsg4), partial CSB-YP757816RA
CSB-EP757816RA
CSB-BP757816RA
CSB-MP757816RA
CSB-EP757816RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Glycerophosphodiester phosphodiesterase domain-containing protein 4 (GDPD4), partial CSB-YP757817HU1
CSB-EP757817HU1
CSB-BP757817HU1
CSB-MP757817HU1
CSB-EP757817HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Neuropeptide S receptor (NPSR1), partial CSB-YP757818HU1
CSB-EP757818HU1
CSB-BP757818HU1
CSB-MP757818HU1
CSB-EP757818HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clethrionomys californicus Cytochrome b (MT-CYB), partial CSB-YP757819CIAF
CSB-EP757819CIAF
CSB-BP757819CIAF
CSB-MP757819CIAF
CSB-EP757819CIAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Horse Aldo-keto reductase family 1 member C23-like protein (PGFS) CSB-YP757820HO
CSB-EP757820HO
CSB-BP757820HO
CSB-MP757820HO
CSB-EP757820HO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Homeobox protein Hox-A13a (hoxa13a) CSB-YP757821DIL
CSB-EP757821DIL
CSB-BP757821DIL
CSB-MP757821DIL
CSB-EP757821DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human metapneumovirus Small hydrophobic protein (SH), partial CSB-YP757822HDAM1
CSB-EP757822HDAM1
CSB-BP757822HDAM1
CSB-MP757822HDAM1
CSB-EP757822HDAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gorilla gorilla gorilla Sulfotransferase 6B1 (SULT6B1) CSB-YP757823GGZ
CSB-EP757823GGZ
CSB-BP757823GGZ
CSB-MP757823GGZ
CSB-EP757823GGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parabuthus granulatus Potassium channel toxin alpha-KTx 11.3 CSB-YP757824ERL
CSB-EP757824ERL
CSB-BP757824ERL
CSB-MP757824ERL
CSB-EP757824ERL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse E3 ubiquitin-protein ligase UBR2 (Ubr2), partial CSB-YP757825MO
CSB-EP757825MO
CSB-BP757825MO
CSB-MP757825MO
CSB-EP757825MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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