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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Cytochrome c biogenesis protein ccsA (ccsA), partial CSB-YP758238AIQ
CSB-EP758238AIQ
CSB-BP758238AIQ
CSB-MP758238AIQ
CSB-EP758238AIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S19, chloroplastic (rps19) CSB-YP758239AIQ
CSB-EP758239AIQ
CSB-BP758239AIQ
CSB-MP758239AIQ
CSB-EP758239AIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S8, chloroplastic (rps8) CSB-YP758240AIQ
CSB-EP758240AIQ
CSB-BP758240AIQ
CSB-MP758240AIQ
CSB-EP758240AIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome b6-f complex subunit 6 (petL), partial CSB-YP758241AIQ
CSB-EP758241AIQ
CSB-BP758241AIQ
CSB-MP758241AIQ
CSB-EP758241AIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta, chloroplastic (accD), partial CSB-YP758242AIQ
CSB-EP758242AIQ
CSB-BP758242AIQ
CSB-MP758242AIQ
CSB-EP758242AIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NAD (P)H-quinone oxidoreductase subunit J, chloroplastic (ndhJ) CSB-YP758243AIQ
CSB-EP758243AIQ
CSB-BP758243AIQ
CSB-MP758243AIQ
CSB-EP758243AIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit c, chloroplastic (atpH), partial CSB-YP758244AIQ1
CSB-EP758244AIQ1
CSB-BP758244AIQ1
CSB-MP758244AIQ1
CSB-EP758244AIQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem Q (B) protein (psbA), partial CSB-YP758245AIQ1
CSB-EP758245AIQ1
CSB-BP758245AIQ1
CSB-MP758245AIQ1
CSB-EP758245AIQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein FRA10AC1 (FRA10AC1) CSB-YP758246HU
CSB-EP758246HU
CSB-BP758246HU
CSB-MP758246HU
CSB-EP758246HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Neuraminidase (NA), partial CSB-YP758247IBV
CSB-EP758247IBV
CSB-BP758247IBV
CSB-MP758247IBV
CSB-EP758247IBV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium funiculosum Histone H4.1 (H4.1) CSB-YP758248PEX
CSB-EP758248PEX
CSB-BP758248PEX
CSB-MP758248PEX
CSB-EP758248PEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Myelin proteolipid protein (PLP1), partial CSB-YP758249PI1
CSB-EP758249PI1
CSB-BP758249PI1
CSB-MP758249PI1
CSB-EP758249PI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gorilla gorilla gorilla Taste receptor type 1 member 3 (TAS1R3), partial CSB-YP758250GGZ
CSB-EP758250GGZ
CSB-BP758250GGZ
CSB-MP758250GGZ
CSB-EP758250GGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein dpy-19 homolog 3 (Dpy19l3), partial CSB-YP758251MO
CSB-EP758251MO
CSB-BP758251MO
CSB-MP758251MO
CSB-EP758251MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ichneumia albicauda Cytochrome b (MT-CYB), partial CSB-YP758252ICAE
CSB-EP758252ICAE
CSB-BP758252ICAE
CSB-MP758252ICAE
CSB-EP758252ICAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crossarchus alexandri Cytochrome b (MT-CYB), partial CSB-YP758253CIAH
CSB-EP758253CIAH
CSB-BP758253CIAH
CSB-MP758253CIAH
CSB-EP758253CIAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse E3 ubiquitin-protein ligase ZNRF2 (Znrf2) CSB-YP758254MO
CSB-EP758254MO
CSB-BP758254MO
CSB-MP758254MO
CSB-EP758254MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein BHLHb9 (Bhlhb9) CSB-YP758255RA
CSB-EP758255RA
CSB-BP758255RA
CSB-MP758255RA
CSB-EP758255RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spirogyra maxima Protein psbN (psbN), partial CSB-YP758256FKM
CSB-EP758256FKM
CSB-BP758256FKM
CSB-MP758256FKM
CSB-EP758256FKM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stangeria eriopus 30S ribosomal protein S7, chloroplastic (rps7) CSB-YP758257SDAC
CSB-EP758257SDAC
CSB-BP758257SDAC
CSB-MP758257SDAC
CSB-EP758257SDAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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