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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Queuine tRNA-ribosyltransferase (tgt) CSB-YP758439LEV
CSB-EP758439LEV
CSB-BP758439LEV
CSB-MP758439LEV
CSB-EP758439LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP758440LEV
CSB-EP758440LEV
CSB-BP758440LEV
CSB-MP758440LEV
CSB-EP758440LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Undecaprenyl pyrophosphate synthase (uppS) CSB-YP758441LEV
CSB-EP758441LEV
CSB-BP758441LEV
CSB-MP758441LEV
CSB-EP758441LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Dihydrodipicolinate reductase (dapB) CSB-YP758442LEV
CSB-EP758442LEV
CSB-BP758442LEV
CSB-MP758442LEV
CSB-EP758442LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 50S ribosomal protein L7/L12 (rplL) CSB-YP758443LEV
CSB-EP758443LEV
CSB-BP758443LEV
CSB-MP758443LEV
CSB-EP758443LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP758444LEV
CSB-EP758444LEV
CSB-BP758444LEV
CSB-MP758444LEV
CSB-EP758444LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS), partial CSB-YP758445LEV
CSB-EP758445LEV
CSB-BP758445LEV
CSB-MP758445LEV
CSB-EP758445LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Phosphoserine aminotransferase (serC) CSB-YP758446LEV
CSB-EP758446LEV
CSB-BP758446LEV
CSB-MP758446LEV
CSB-EP758446LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB) CSB-YP758447LEV
CSB-EP758447LEV
CSB-BP758447LEV
CSB-MP758447LEV
CSB-EP758447LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 50S ribosomal protein L34 (rpmH) CSB-YP758448LEV
CSB-EP758448LEV
CSB-BP758448LEV
CSB-MP758448LEV
CSB-EP758448LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Maf-like protein LIC_10016 (LIC_10016) CSB-YP758449LEV
CSB-EP758449LEV
CSB-BP758449LEV
CSB-MP758449LEV
CSB-EP758449LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S6 (rpsF) CSB-YP758450BQN
CSB-EP758450BQN
CSB-BP758450BQN
CSB-MP758450BQN
CSB-EP758450BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus FMN-dependent NADH-azoreductase 4 (azoR4) CSB-YP758451BQN
CSB-EP758451BQN
CSB-BP758451BQN
CSB-MP758451BQN
CSB-EP758451BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UV DNA damage endonuclease (uvsE) CSB-YP758452BQN
CSB-EP758452BQN
CSB-BP758452BQN
CSB-MP758452BQN
CSB-EP758452BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP synthase subunit c (atpE), partial CSB-YP758453BQN1
CSB-EP758453BQN1
CSB-BP758453BQN1
CSB-MP758453BQN1
CSB-EP758453BQN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP synthase gamma chain (atpG) CSB-YP758454BQN
CSB-EP758454BQN
CSB-BP758454BQN
CSB-MP758454BQN
CSB-EP758454BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0042 nucleotide-binding protein BCE_5259 (BCE_5259) CSB-YP758455BQN
CSB-EP758455BQN
CSB-BP758455BQN
CSB-MP758455BQN
CSB-EP758455BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Protein psiE homolog (psiE), partial CSB-YP758456BQN1
CSB-EP758456BQN1
CSB-BP758456BQN1
CSB-MP758456BQN1
CSB-EP758456BQN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methionine import ATP-binding protein MetN 3 (metN3) CSB-YP758457BQN
CSB-EP758457BQN
CSB-BP758457BQN
CSB-MP758457BQN
CSB-EP758457BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glycine--tRNA ligase (glyQS) CSB-YP758458BQN
CSB-EP758458BQN
CSB-BP758458BQN
CSB-MP758458BQN
CSB-EP758458BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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