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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP756764SKW
CSB-EP756764SKW
CSB-BP756764SKW
CSB-MP756764SKW
CSB-EP756764SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L21 (rplU) CSB-YP756765SKW
CSB-EP756765SKW
CSB-BP756765SKW
CSB-MP756765SKW
CSB-EP756765SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus D-tyrosyl-tRNA (Tyr) deacylase (dtd) CSB-YP756766SKW
CSB-EP756766SKW
CSB-BP756766SKW
CSB-MP756766SKW
CSB-EP756766SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative Holliday junction resolvase (SAS1552) CSB-YP756767SKW
CSB-EP756767SKW
CSB-BP756767SKW
CSB-MP756767SKW
CSB-EP756767SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein grpE (grpE) CSB-YP756768SKW
CSB-EP756768SKW
CSB-BP756768SKW
CSB-MP756768SKW
CSB-EP756768SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus RNA polymerase sigma factor rpoD (rpoD) CSB-YP756769SKW
CSB-EP756769SKW
CSB-BP756769SKW
CSB-MP756769SKW
CSB-EP756769SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP756770SKW
CSB-EP756770SKW
CSB-BP756770SKW
CSB-MP756770SKW
CSB-EP756770SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Pyrroline-5-carboxylate reductase (proC) CSB-YP756771SKW
CSB-EP756771SKW
CSB-BP756771SKW
CSB-MP756771SKW
CSB-EP756771SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Segregation and condensation protein B (scpB) CSB-YP756772SKW
CSB-EP756772SKW
CSB-BP756772SKW
CSB-MP756772SKW
CSB-EP756772SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S1 (rpsA) CSB-YP756773SKW
CSB-EP756773SKW
CSB-BP756773SKW
CSB-MP756773SKW
CSB-EP756773SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Asparagine--tRNA ligase (asnS) CSB-YP756774SKW
CSB-EP756774SKW
CSB-BP756774SKW
CSB-MP756774SKW
CSB-EP756774SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Alanine dehydrogenase 1 (ald1) CSB-YP756775SKW
CSB-EP756775SKW
CSB-BP756775SKW
CSB-MP756775SKW
CSB-EP756775SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DegV domain-containing protein SAS1368 (SAS1368) CSB-YP756776SKW
CSB-EP756776SKW
CSB-BP756776SKW
CSB-MP756776SKW
CSB-EP756776SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Response regulator ArlR (arlR) CSB-YP756777SKW
CSB-EP756777SKW
CSB-BP756777SKW
CSB-MP756777SKW
CSB-EP756777SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative oligopeptide transport ATP-binding protein oppD2 (oppD2) CSB-YP756778SKW
CSB-EP756778SKW
CSB-BP756778SKW
CSB-MP756778SKW
CSB-EP756778SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Aminoacyltransferase FemB (femB) CSB-YP756779SKW
CSB-EP756779SKW
CSB-BP756779SKW
CSB-MP756779SKW
CSB-EP756779SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Tryptophan synthase beta chain (trpB) CSB-YP756780SKW
CSB-EP756780SKW
CSB-BP756780SKW
CSB-MP756780SKW
CSB-EP756780SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Peptide methionine sulfoxide reductase MsrA 1 (msrA1) CSB-YP756781SKW
CSB-EP756781SKW
CSB-BP756781SKW
CSB-MP756781SKW
CSB-EP756781SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA topoisomerase 4 subunit B (parE), partial CSB-YP756782SKW
CSB-EP756782SKW
CSB-BP756782SKW
CSB-MP756782SKW
CSB-EP756782SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S14 (rpsN) CSB-YP756783SKW
CSB-EP756783SKW
CSB-BP756783SKW
CSB-MP756783SKW
CSB-EP756783SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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