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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermus thermophilus S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP758399TNS
CSB-EP758399TNS
CSB-BP758399TNS
CSB-MP758399TNS
CSB-EP758399TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Triosephosphate isomerase (tpiA) CSB-YP758400TNS
CSB-EP758400TNS
CSB-BP758400TNS
CSB-MP758400TNS
CSB-EP758400TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Cysteine--tRNA ligase (cysS) CSB-YP758401TNS
CSB-EP758401TNS
CSB-BP758401TNS
CSB-MP758401TNS
CSB-EP758401TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP758402TNS
CSB-EP758402TNS
CSB-BP758402TNS
CSB-MP758402TNS
CSB-EP758402TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Aspartate carbamoyltransferase (pyrB) CSB-YP758403TNS
CSB-EP758403TNS
CSB-BP758403TNS
CSB-MP758403TNS
CSB-EP758403TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP758404TNS
CSB-EP758404TNS
CSB-BP758404TNS
CSB-MP758404TNS
CSB-EP758404TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus UPF0340 protein TT_C0214 (TT_C0214) CSB-YP758405TNS
CSB-EP758405TNS
CSB-BP758405TNS
CSB-MP758405TNS
CSB-EP758405TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Cytidylate kinase (cmk) CSB-YP758406TNS
CSB-EP758406TNS
CSB-BP758406TNS
CSB-MP758406TNS
CSB-EP758406TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP758407TNS
CSB-EP758407TNS
CSB-BP758407TNS
CSB-MP758407TNS
CSB-EP758407TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Recombination protein RecR (recR) CSB-YP758408LEV
CSB-EP758408LEV
CSB-BP758408LEV
CSB-MP758408LEV
CSB-EP758408LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Acetyl-coenzyme A synthetase (acsA), partial CSB-YP758409LEV
CSB-EP758409LEV
CSB-BP758409LEV
CSB-MP758409LEV
CSB-EP758409LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Macro domain-containing protein LIC_13295 (LIC_13295) CSB-YP758410LEV
CSB-EP758410LEV
CSB-BP758410LEV
CSB-MP758410LEV
CSB-EP758410LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP758411LEV
CSB-EP758411LEV
CSB-BP758411LEV
CSB-MP758411LEV
CSB-EP758411LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 5'-nucleotidase surE (surE) CSB-YP758412LEV
CSB-EP758412LEV
CSB-BP758412LEV
CSB-MP758412LEV
CSB-EP758412LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 50S ribosomal protein L3 (rplC) CSB-YP758413LEV
CSB-EP758413LEV
CSB-BP758413LEV
CSB-MP758413LEV
CSB-EP758413LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 50S ribosomal protein L14 (rplN) CSB-YP758414LEV
CSB-EP758414LEV
CSB-BP758414LEV
CSB-MP758414LEV
CSB-EP758414LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 50S ribosomal protein L5 (rplE) CSB-YP758415LEV
CSB-EP758415LEV
CSB-BP758415LEV
CSB-MP758415LEV
CSB-EP758415LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 50S ribosomal protein L6 (rplF) CSB-YP758416LEV
CSB-EP758416LEV
CSB-BP758416LEV
CSB-MP758416LEV
CSB-EP758416LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 30S ribosomal protein S4 (rpsD) CSB-YP758417LEV
CSB-EP758417LEV
CSB-BP758417LEV
CSB-MP758417LEV
CSB-EP758417LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP758418LEV
CSB-EP758418LEV
CSB-BP758418LEV
CSB-MP758418LEV
CSB-EP758418LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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