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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bdellovibrio bacteriovorus Probable potassium transport system protein kup (kup), partial CSB-YP757365BFP
CSB-EP757365BFP
CSB-BP757365BFP
CSB-MP757365BFP
CSB-EP757365BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Methionine--tRNA ligase (metG), partial CSB-YP757366BFP
CSB-EP757366BFP
CSB-BP757366BFP
CSB-MP757366BFP
CSB-EP757366BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus D-tyrosyl-tRNA (Tyr) deacylase (dtd) CSB-YP757367BFP
CSB-EP757367BFP
CSB-BP757367BFP
CSB-MP757367BFP
CSB-EP757367BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Ribosome maturation factor RimP (rimP) CSB-YP757368BFP
CSB-EP757368BFP
CSB-BP757368BFP
CSB-MP757368BFP
CSB-EP757368BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus UPF0324 membrane protein Bd1437 (Bd1437), partial CSB-YP757369BFP
CSB-EP757369BFP
CSB-BP757369BFP
CSB-MP757369BFP
CSB-EP757369BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP757370BFP
CSB-EP757370BFP
CSB-BP757370BFP
CSB-MP757370BFP
CSB-EP757370BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Triosephosphate isomerase (tpiA) CSB-YP757371BFP
CSB-EP757371BFP
CSB-BP757371BFP
CSB-MP757371BFP
CSB-EP757371BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP757372BFP
CSB-EP757372BFP
CSB-BP757372BFP
CSB-MP757372BFP
CSB-EP757372BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP757373BFP
CSB-EP757373BFP
CSB-BP757373BFP
CSB-MP757373BFP
CSB-EP757373BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Error-prone DNA polymerase (dnaE2), partial CSB-YP757374BFP
CSB-EP757374BFP
CSB-BP757374BFP
CSB-MP757374BFP
CSB-EP757374BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP757375BFP
CSB-EP757375BFP
CSB-BP757375BFP
CSB-MP757375BFP
CSB-EP757375BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Peptide chain release factor 1 (prfA) CSB-YP757376BFP
CSB-EP757376BFP
CSB-BP757376BFP
CSB-MP757376BFP
CSB-EP757376BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Chromosomal replication initiator protein DnaA (dnaA) CSB-YP757377BFP
CSB-EP757377BFP
CSB-BP757377BFP
CSB-MP757377BFP
CSB-EP757377BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 50S ribosomal protein L7/L12 (rplL) CSB-YP757378MLS
CSB-EP757378MLS
CSB-BP757378MLS
CSB-MP757378MLS
CSB-EP757378MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Deoxyribose-phosphate aldolase (deoC) CSB-YP757379MLS
CSB-EP757379MLS
CSB-BP757379MLS
CSB-MP757379MLS
CSB-EP757379MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 30S ribosomal protein S10 (rpsJ) CSB-YP757380MLS
CSB-EP757380MLS
CSB-BP757380MLS
CSB-MP757380MLS
CSB-EP757380MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 50S ribosomal protein L14 (rplN) CSB-YP757381MLS
CSB-EP757381MLS
CSB-BP757381MLS
CSB-MP757381MLS
CSB-EP757381MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 50S ribosomal protein L17 (rplQ) CSB-YP757382MLS
CSB-EP757382MLS
CSB-BP757382MLS
CSB-MP757382MLS
CSB-EP757382MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Leucine--tRNA ligase (leuS), partial CSB-YP757383MLS
CSB-EP757383MLS
CSB-BP757383MLS
CSB-MP757383MLS
CSB-EP757383MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP757384MLS
CSB-EP757384MLS
CSB-BP757384MLS
CSB-MP757384MLS
CSB-EP757384MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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