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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mouse 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial (Bckdhb) CSB-YP757565MO
CSB-EP757565MO
CSB-BP757565MO
CSB-MP757565MO
CSB-EP757565MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Nicotinate phosphoribosyltransferase (naprt1), partial CSB-YP757566DIL
CSB-EP757566DIL
CSB-BP757566DIL
CSB-MP757566DIL
CSB-EP757566DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Zinc transporter 7 (slc30a7), partial CSB-YP757567XBF1
CSB-EP757567XBF1
CSB-BP757567XBF1
CSB-MP757567XBF1
CSB-EP757567XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human DENN domain-containing protein 1B (DENND1B) CSB-YP757568HU
CSB-EP757568HU
CSB-BP757568HU
CSB-MP757568HU
CSB-EP757568HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tetratricopeptide repeat protein 27 (TTC27), partial CSB-YP757569HU
CSB-EP757569HU
CSB-BP757569HU
CSB-MP757569HU
CSB-EP757569HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Mediator of RNA polymerase II transcription subunit 23 (med23), partial CSB-YP757570XBE
CSB-EP757570XBE
CSB-BP757570XBE
CSB-MP757570XBE
CSB-EP757570XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein FAM122C (FAM122C) CSB-YP757571HU
CSB-EP757571HU
CSB-BP757571HU
CSB-MP757571HU
CSB-EP757571HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 (pin4) CSB-YP757572XBF
CSB-EP757572XBF
CSB-BP757572XBF
CSB-MP757572XBF
CSB-EP757572XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Nuclear factor related to kappa-B-binding protein (NFRKB), partial CSB-YP757573HU
CSB-EP757573HU
CSB-BP757573HU
CSB-MP757573HU
CSB-EP757573HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Synembryn-A (ric8a), partial CSB-YP757574XBF
CSB-EP757574XBF
CSB-BP757574XBF
CSB-MP757574XBF
CSB-EP757574XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat COP9 signalosome complex subunit 8 (Cops8) CSB-YP757575RA
CSB-EP757575RA
CSB-BP757575RA
CSB-MP757575RA
CSB-EP757575RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse RRP12-like protein (Rrp12), partial CSB-YP757576MO
CSB-EP757576MO
CSB-BP757576MO
CSB-MP757576MO
CSB-EP757576MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein tweety homolog 3 (Ttyh3), partial CSB-YP757577MO1
CSB-EP757577MO1
CSB-BP757577MO1
CSB-MP757577MO1
CSB-EP757577MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Arginine and glutamate-rich protein 1-A (arglu1a) CSB-YP757578DIL
CSB-EP757578DIL
CSB-BP757578DIL
CSB-MP757578DIL
CSB-EP757578DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Coiled-coil domain-containing protein 148 (Ccdc148) CSB-YP757579MO
CSB-EP757579MO
CSB-BP757579MO
CSB-MP757579MO
CSB-EP757579MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Adenylate kinase 8 (ak8) CSB-YP757580XBF
CSB-EP757580XBF
CSB-BP757580XBF
CSB-MP757580XBF
CSB-EP757580XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Plastin-2 (lcp1), partial CSB-YP757581DIL
CSB-EP757581DIL
CSB-BP757581DIL
CSB-MP757581DIL
CSB-EP757581DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein YIPF1 (Yipf1), partial CSB-YP757582RA1
CSB-EP757582RA1
CSB-BP757582RA1
CSB-MP757582RA1
CSB-EP757582RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat mTERF domain-containing protein 1, mitochondrial (Mterfd1) CSB-YP757583RA
CSB-EP757583RA
CSB-BP757583RA
CSB-MP757583RA
CSB-EP757583RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Nucleotide exchange factor SIL1 (Sil1) CSB-YP757584RA
CSB-EP757584RA
CSB-BP757584RA
CSB-MP757584RA
CSB-EP757584RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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