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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP758459BQN
CSB-EP758459BQN
CSB-BP758459BQN
CSB-MP758459BQN
CSB-EP758459BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Large-conductance mechanosensitive channel (mscL), partial CSB-YP758460BQN1
CSB-EP758460BQN1
CSB-BP758460BQN1
CSB-MP758460BQN1
CSB-EP758460BQN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Septation ring formation regulator EzrA (ezrA), partial CSB-YP758461BQN
CSB-EP758461BQN
CSB-BP758461BQN
CSB-MP758461BQN
CSB-EP758461BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Transcriptional repressor NrdR (nrdR) CSB-YP758462BQN
CSB-EP758462BQN
CSB-BP758462BQN
CSB-MP758462BQN
CSB-EP758462BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP758463BQN
CSB-EP758463BQN
CSB-BP758463BQN
CSB-MP758463BQN
CSB-EP758463BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribonuclease HIII (rnhC) CSB-YP758464BQN
CSB-EP758464BQN
CSB-BP758464BQN
CSB-MP758464BQN
CSB-EP758464BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Trigger factor (tig) CSB-YP758465BQN
CSB-EP758465BQN
CSB-BP758465BQN
CSB-MP758465BQN
CSB-EP758465BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Septum formation protein Maf (maf) CSB-YP758466BQN
CSB-EP758466BQN
CSB-BP758466BQN
CSB-MP758466BQN
CSB-EP758466BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP758467BQN
CSB-EP758467BQN
CSB-BP758467BQN
CSB-MP758467BQN
CSB-EP758467BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Uncharacterized methyltransferase BCE_4457 (BCE_4457) CSB-YP758468BQN
CSB-EP758468BQN
CSB-BP758468BQN
CSB-MP758468BQN
CSB-EP758468BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S20 (rpsT) CSB-YP758469BQN
CSB-EP758469BQN
CSB-BP758469BQN
CSB-MP758469BQN
CSB-EP758469BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus DNA repair protein recO (recO) CSB-YP758470BQN
CSB-EP758470BQN
CSB-BP758470BQN
CSB-MP758470BQN
CSB-EP758470BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Bifunctional protein FolD (folD) CSB-YP758471BQN
CSB-EP758471BQN
CSB-BP758471BQN
CSB-MP758471BQN
CSB-EP758471BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Biotin synthase (bioB) CSB-YP758472BQN
CSB-EP758472BQN
CSB-BP758472BQN
CSB-MP758472BQN
CSB-EP758472BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Segregation and condensation protein B (scpB) CSB-YP758473BQN
CSB-EP758473BQN
CSB-BP758473BQN
CSB-MP758473BQN
CSB-EP758473BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2,3-diketo-5-methylthiopentyl-1-phosphate enolase (mtnW) CSB-YP758474BQN
CSB-EP758474BQN
CSB-BP758474BQN
CSB-MP758474BQN
CSB-EP758474BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (dapH) CSB-YP758475BQN
CSB-EP758475BQN
CSB-BP758475BQN
CSB-MP758475BQN
CSB-EP758475BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0358 protein BCE_3996 (BCE_3996) CSB-YP758476BQN
CSB-EP758476BQN
CSB-BP758476BQN
CSB-MP758476BQN
CSB-EP758476BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Bifunctional protein pyrR (pyrR) CSB-YP758477BQN
CSB-EP758477BQN
CSB-BP758477BQN
CSB-MP758477BQN
CSB-EP758477BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Dihydroorotate dehydrogenase B (NAD (+)), catalytic subunit (pyrD) CSB-YP758478BQN
CSB-EP758478BQN
CSB-BP758478BQN
CSB-MP758478BQN
CSB-EP758478BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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