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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus thuringiensis subsp. konkukian Hydroxyethylthiazole kinase (thiM) CSB-YP757065BAAE
CSB-EP757065BAAE
CSB-BP757065BAAE
CSB-MP757065BAAE
CSB-EP757065BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Ferredoxin--NADP reductase 1 (BT9727_0321) CSB-YP757066BAAE
CSB-EP757066BAAE
CSB-BP757066BAAE
CSB-MP757066BAAE
CSB-EP757066BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Heptaprenylglyceryl phosphate synthase (pcrB) CSB-YP757067BAAE
CSB-EP757067BAAE
CSB-BP757067BAAE
CSB-MP757067BAAE
CSB-EP757067BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Undecaprenyl-diphosphatase 1 (uppP1), partial CSB-YP757068BAAE1
CSB-EP757068BAAE1
CSB-BP757068BAAE1
CSB-MP757068BAAE1
CSB-EP757068BAAE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP757069BAAE
CSB-EP757069BAAE
CSB-BP757069BAAE
CSB-MP757069BAAE
CSB-EP757069BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L17 (rplQ) CSB-YP757070BAAE
CSB-EP757070BAAE
CSB-BP757070BAAE
CSB-MP757070BAAE
CSB-EP757070BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L15 (rplO) CSB-YP757071BAAE
CSB-EP757071BAAE
CSB-BP757071BAAE
CSB-MP757071BAAE
CSB-EP757071BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 30S ribosomal protein S8 (rpsH) CSB-YP757072BAAE
CSB-EP757072BAAE
CSB-BP757072BAAE
CSB-MP757072BAAE
CSB-EP757072BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 30S ribosomal protein S3 (rpsC) CSB-YP757073BAAE
CSB-EP757073BAAE
CSB-BP757073BAAE
CSB-MP757073BAAE
CSB-EP757073BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Elongation factor Tu (tuf) CSB-YP757074BAAE
CSB-EP757074BAAE
CSB-BP757074BAAE
CSB-MP757074BAAE
CSB-EP757074BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L7/L12 (rplL) CSB-YP757075BAAE
CSB-EP757075BAAE
CSB-BP757075BAAE
CSB-MP757075BAAE
CSB-EP757075BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Glutamate--tRNA ligase (gltX) CSB-YP757076BAAE
CSB-EP757076BAAE
CSB-BP757076BAAE
CSB-MP757076BAAE
CSB-EP757076BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Putative ATP:guanido phosphotransferase BT9727_0077 (karG) CSB-YP757077BAAE
CSB-EP757077BAAE
CSB-BP757077BAAE
CSB-MP757077BAAE
CSB-EP757077BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian tRNA (Ile)-lysidine synthase (tilS) CSB-YP757078BAAE
CSB-EP757078BAAE
CSB-BP757078BAAE
CSB-MP757078BAAE
CSB-EP757078BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Initiation-control protein yabA (BT9727_0029) CSB-YP757079BAAE
CSB-EP757079BAAE
CSB-BP757079BAAE
CSB-MP757079BAAE
CSB-EP757079BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrophosphatase ppaX (ppaX) CSB-YP757080BQE
CSB-EP757080BQE
CSB-BP757080BQE
CSB-MP757080BQE
CSB-EP757080BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable protein ABIL4 (Os05g0585400, LOC_Os05g50800) CSB-YP757081OFG
CSB-EP757081OFG
CSB-BP757081OFG
CSB-MP757081OFG
CSB-EP757081OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cynops pyrrhogaster Fibroblast growth factor 1 (fgf1) CSB-YP757082EQC
CSB-EP757082EQC
CSB-BP757082EQC
CSB-MP757082EQC
CSB-EP757082EQC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ragulator complex protein LAMTOR1 (LAMTOR1) CSB-YP757083HU
CSB-BP757083HU
CSB-MP757083HU
CSB-EP757083HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Desumoylating isopeptidase 1 (PPPDE2) CSB-YP757084HU
CSB-EP757084HU
CSB-BP757084HU
CSB-MP757084HU
CSB-EP757084HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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