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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Stangeria eriopus Photosystem II reaction center protein L (psbL), partial CSB-YP758258SDAC
CSB-EP758258SDAC
CSB-BP758258SDAC
CSB-MP758258SDAC
CSB-EP758258SDAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cycas revoluta Photosystem II reaction center protein L (psbL), partial CSB-YP758259EPY
CSB-EP758259EPY
CSB-BP758259EPY
CSB-MP758259EPY
CSB-EP758259EPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem II reaction center protein T (psbT), partial CSB-YP758260MES
CSB-EP758260MES
CSB-BP758260MES
CSB-MP758260MES
CSB-EP758260MES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Xin actin-binding repeat-containing protein 2 (Xirp2), partial CSB-YP758261RA
CSB-EP758261RA
CSB-BP758261RA
CSB-MP758261RA
CSB-EP758261RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dasyprocta fuliginosa Cytochrome b (MT-CYB), partial CSB-YP758262DCAR
CSB-EP758262DCAR
CSB-BP758262DCAR
CSB-MP758262DCAR
CSB-EP758262DCAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Lecithine-cholesterol acyltransferase-like 4 (LCAT4), partial CSB-YP758263DOA
CSB-EP758263DOA
CSB-BP758263DOA
CSB-MP758263DOA
CSB-EP758263DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viridovipera stejnegeri Snake venom serine protease KN8 CSB-YP758264VDN
CSB-EP758264VDN
CSB-BP758264VDN
CSB-MP758264VDN
CSB-EP758264VDN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viridovipera stejnegeri Snake venom serine protease KN1 CSB-YP758265VDN
CSB-EP758265VDN
CSB-BP758265VDN
CSB-MP758265VDN
CSB-EP758265VDN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human La-related protein 4 (LARP4) CSB-YP758266HU
CSB-EP758266HU
CSB-BP758266HU
CSB-MP758266HU
CSB-EP758266HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viridovipera stejnegeri Coagulation factor IX/factor X-binding protein subunit A CSB-YP758267VDN
CSB-EP758267VDN
CSB-BP758267VDN
CSB-MP758267VDN
CSB-EP758267VDN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca mulatta Cannabinoid receptor 1 (CNR1), partial CSB-YP758268MOW1
CSB-EP758268MOW1
CSB-BP758268MOW1
CSB-MP758268MOW1
CSB-EP758268MOW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Euphorbia esula Histone H3.2 (H3) CSB-YP758269EIG
CSB-EP758269EIG
CSB-BP758269EIG
CSB-MP758269EIG
CSB-EP758269EIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza coarctata Histone H3.3 CSB-YP758270OBAI
CSB-EP758270OBAI
CSB-BP758270OBAI
CSB-MP758270OBAI
CSB-EP758270OBAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sminthopsis douglasi Sperm protamine P1 (PRM1) CSB-YP758271SFAJ
CSB-EP758271SFAJ
CSB-BP758271SFAJ
CSB-MP758271SFAJ
CSB-EP758271SFAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human 40S ribosomal protein S27-like (RPS27L) CSB-YP758272HU
CSB-EP758272HU
CSB-BP758272HU
CSB-MP758272HU
CSB-EP758272HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murexia rothschildi Sperm protamine P1 (PRM1) CSB-YP758273MBAW
CSB-EP758273MBAW
CSB-BP758273MBAW
CSB-MP758273MBAW
CSB-EP758273MBAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudantechinus woolleyae Sperm protamine P1 (PRM1) CSB-YP758274PDAC
CSB-EP758274PDAC
CSB-BP758274PDAC
CSB-MP758274PDAC
CSB-EP758274PDAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ningaui yvonnae Sperm protamine P1 (PRM1) CSB-YP758275NAAT
CSB-EP758275NAAT
CSB-BP758275NAAT
CSB-MP758275NAAT
CSB-EP758275NAAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Rhamnulose-1-phosphate aldolase (rhaD) CSB-YP758276LAAD
CSB-EP758276LAAD
CSB-BP758276LAAD
CSB-MP758276LAAD
CSB-EP758276LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Probable transaldolase 1 (tal1) CSB-YP758277LAAD
CSB-EP758277LAAD
CSB-BP758277LAAD
CSB-MP758277LAAD
CSB-EP758277LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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