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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus 50S ribosomal protein L18 (rplR) CSB-YP758920SKY
CSB-EP758920SKY
CSB-BP758920SKY
CSB-MP758920SKY
CSB-EP758920SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Pyrimidine-nucleoside phosphorylase (pdp) CSB-YP758921SKY
CSB-EP758921SKY
CSB-BP758921SKY
CSB-MP758921SKY
CSB-EP758921SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable transglycosylase sceD (sceD) CSB-YP758922SKY
CSB-EP758922SKY
CSB-BP758922SKY
CSB-MP758922SKY
CSB-EP758922SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP758923SKY
CSB-EP758923SKY
CSB-BP758923SKY
CSB-MP758923SKY
CSB-EP758923SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0421 protein SA1705 (SA1705), partial CSB-YP758924SKY1
CSB-EP758924SKY1
CSB-BP758924SKY1
CSB-MP758924SKY1
CSB-EP758924SKY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative multidrug export ATP-binding/permease protein SA1683 (SA1683), partial CSB-YP758925SKY1
CSB-EP758925SKY1
CSB-BP758925SKY1
CSB-MP758925SKY1
CSB-EP758925SKY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine protease splB (splB) CSB-YP758926SKY
CSB-EP758926SKY
CSB-BP758926SKY
CSB-MP758926SKY
CSB-EP758926SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0354 protein SA1564 (SA1564) CSB-YP758927SKY
CSB-EP758927SKY
CSB-BP758927SKY
CSB-MP758927SKY
CSB-EP758927SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP758928SKY
CSB-EP758928SKY
CSB-BP758928SKY
CSB-MP758928SKY
CSB-EP758928SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0403 protein SA1345 (SA1345) CSB-YP758929SKY
CSB-EP758929SKY
CSB-BP758929SKY
CSB-MP758929SKY
CSB-EP758929SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA-binding protein HU (hup) CSB-YP758930SKY
CSB-EP758930SKY
CSB-BP758930SKY
CSB-MP758930SKY
CSB-EP758930SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Extracellular matrix-binding protein ebhB (ebhB), partial CSB-YP758931SKY
CSB-EP758931SKY
CSB-BP758931SKY
CSB-MP758931SKY
CSB-EP758931SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Cold shock protein CspA (cspA) CSB-YP758932SKY
CSB-EP758932SKY
CSB-BP758932SKY
CSB-MP758932SKY
CSB-EP758932SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative oligopeptide transport ATP-binding protein oppD2 (oppD2) CSB-YP758933SKY
CSB-EP758933SKY
CSB-BP758933SKY
CSB-MP758933SKY
CSB-EP758933SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Catalase (katA), partial CSB-YP758934SKY
CSB-EP758934SKY
CSB-BP758934SKY
CSB-MP758934SKY
CSB-EP758934SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable cell wall hydrolase lytN (lytN) CSB-YP758935SKY
CSB-EP758935SKY
CSB-BP758935SKY
CSB-MP758935SKY
CSB-EP758935SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Carbamate kinase 1 (arcC1) CSB-YP758936SKY
CSB-EP758936SKY
CSB-BP758936SKY
CSB-MP758936SKY
CSB-EP758936SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0348 protein SA0974 (SA0974) CSB-YP758937SKY
CSB-EP758937SKY
CSB-BP758937SKY
CSB-MP758937SKY
CSB-EP758937SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus N5-carboxyaminoimidazole ribonucleotide synthase (purK) CSB-YP758938SKY
CSB-EP758938SKY
CSB-BP758938SKY
CSB-MP758938SKY
CSB-EP758938SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 1,4-Dihydroxy-2-naphthoyl-CoA synthase (menB) CSB-YP758939SKY
CSB-EP758939SKY
CSB-BP758939SKY
CSB-MP758939SKY
CSB-EP758939SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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