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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Photobacterium profundum Regulator of ribonuclease activity B (rraB) CSB-YP757265PIG
CSB-EP757265PIG
CSB-BP757265PIG
CSB-MP757265PIG
CSB-EP757265PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP757266PIG
CSB-EP757266PIG
CSB-BP757266PIG
CSB-MP757266PIG
CSB-EP757266PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum RNA polymerase-associated protein RapA (rapA), partial CSB-YP757267PIG
CSB-EP757267PIG
CSB-BP757267PIG
CSB-MP757267PIG
CSB-EP757267PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Bis (5'-nucleosyl)-tetraphosphatase, symmetrical (apaH) CSB-YP757268PIG
CSB-EP757268PIG
CSB-BP757268PIG
CSB-MP757268PIG
CSB-EP757268PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 30S ribosomal protein S14 (rpsN) CSB-YP757269PIG
CSB-EP757269PIG
CSB-BP757269PIG
CSB-MP757269PIG
CSB-EP757269PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 50S ribosomal protein L16 (rplP) CSB-YP757270PIG
CSB-EP757270PIG
CSB-BP757270PIG
CSB-MP757270PIG
CSB-EP757270PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 30S ribosomal protein S10 (rpsJ) CSB-YP757271PIG
CSB-EP757271PIG
CSB-BP757271PIG
CSB-MP757271PIG
CSB-EP757271PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP757272PIG
CSB-EP757272PIG
CSB-BP757272PIG
CSB-MP757272PIG
CSB-EP757272PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 6-phosphofructokinase (pfkA) CSB-YP757273PIG
CSB-EP757273PIG
CSB-BP757273PIG
CSB-MP757273PIG
CSB-EP757273PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 3-deoxy-D-manno-octulosonic acid kinase (kdkA) CSB-YP757274PIG
CSB-EP757274PIG
CSB-BP757274PIG
CSB-MP757274PIG
CSB-EP757274PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 50S ribosomal protein L28 (rpmB) CSB-YP757275PIG
CSB-EP757275PIG
CSB-BP757275PIG
CSB-MP757275PIG
CSB-EP757275PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Guanylate kinase (gmk) CSB-YP757276PIG
CSB-EP757276PIG
CSB-BP757276PIG
CSB-MP757276PIG
CSB-EP757276PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Probable chorismate--pyruvate lyase (ubiC) CSB-YP757277PIG
CSB-EP757277PIG
CSB-BP757277PIG
CSB-MP757277PIG
CSB-EP757277PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Sec-independent protein translocase protein TatA (tatA), partial CSB-YP757278PIG
CSB-EP757278PIG
CSB-BP757278PIG
CSB-MP757278PIG
CSB-EP757278PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Fatty acid oxidation complex subunit alpha (fadB), partial CSB-YP757279PIG
CSB-EP757279PIG
CSB-BP757279PIG
CSB-MP757279PIG
CSB-EP757279PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Membrane protein insertase YidC (yidC), partial CSB-YP757280PIG1
CSB-EP757280PIG1
CSB-BP757280PIG1
CSB-MP757280PIG1
CSB-EP757280PIG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis DNA polymerase sliding clamp (pcn) CSB-YP757281MSB
CSB-EP757281MSB
CSB-BP757281MSB
CSB-MP757281MSB
CSB-EP757281MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Carbamoyl-phosphate synthase small chain (carA) CSB-YP757282MSB
CSB-EP757282MSB
CSB-BP757282MSB
CSB-MP757282MSB
CSB-EP757282MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Nicotinamide-nucleotide adenylyltransferase (MMP1578) CSB-YP757283MSB
CSB-EP757283MSB
CSB-BP757283MSB
CSB-MP757283MSB
CSB-EP757283MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Tetrahydromethanopterin S-methyltransferase subunit D (mtrD), partial CSB-YP757284MSB1
CSB-EP757284MSB1
CSB-BP757284MSB1
CSB-MP757284MSB1
CSB-EP757284MSB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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