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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Probable quinol oxidase subunit 4 (qoxD), partial CSB-YP758899SUL1
CSB-EP758899SUL1
CSB-BP758899SUL1
CSB-MP758899SUL1
CSB-EP758899SUL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative peptidyl-prolyl cis-trans isomerase (MW0836) CSB-YP758900SUL
CSB-EP758900SUL
CSB-BP758900SUL
CSB-MP758900SUL
CSB-EP758900SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative acetyltransferase MW0724 (MW0724) CSB-YP758901SUL
CSB-EP758901SUL
CSB-BP758901SUL
CSB-MP758901SUL
CSB-EP758901SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Histidine protein kinase saeS (saeS), partial CSB-YP758902SUL1
CSB-EP758902SUL1
CSB-BP758902SUL1
CSB-MP758902SUL1
CSB-EP758902SUL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0741 protein MW0548 (MW0548) CSB-YP758903SUL
CSB-EP758903SUL
CSB-BP758903SUL
CSB-MP758903SUL
CSB-EP758903SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Transcriptional regulator CtsR (ctsR) CSB-YP758904SUL
CSB-EP758904SUL
CSB-BP758904SUL
CSB-MP758904SUL
CSB-EP758904SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus D-ribose pyranase (rbsD) CSB-YP758905SUL
CSB-EP758905SUL
CSB-BP758905SUL
CSB-MP758905SUL
CSB-EP758905SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphonates import ATP-binding protein PhnC (phnC) CSB-YP758906SUL
CSB-EP758906SUL
CSB-BP758906SUL
CSB-MP758906SUL
CSB-EP758906SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Immunodominant staphylococcal antigen B (isaB) CSB-YP758907SKX
CSB-EP758907SKX
CSB-BP758907SKX
CSB-MP758907SKX
CSB-EP758907SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Response regulator protein vraR (vraR) CSB-YP758908SKX
CSB-EP758908SKX
CSB-BP758908SKX
CSB-MP758908SKX
CSB-EP758908SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Signal transduction histidine-protein kinase ArlS (arlS), partial CSB-YP758909SKX1
CSB-EP758909SKX1
CSB-BP758909SKX1
CSB-MP758909SKX1
CSB-EP758909SKX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized membrane protein SAV0746 (SAV0746), partial CSB-YP758910SKX1
CSB-EP758910SKX1
CSB-BP758910SKX1
CSB-MP758910SKX1
CSB-EP758910SKX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Lactonase drp35 (drp35) CSB-YP758911SKY
CSB-EP758911SKY
CSB-BP758911SKY
CSB-MP758911SKY
CSB-EP758911SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine-rich adhesin for platelets (sraP), partial CSB-YP758912SKY
CSB-EP758912SKY
CSB-BP758912SKY
CSB-MP758912SKY
CSB-EP758912SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative acetyltransferase SA2342 (SA2342) CSB-YP758914SKY
CSB-EP758914SKY
CSB-BP758914SKY
CSB-MP758914SKY
CSB-EP758914SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UTP--glucose-1-phosphate uridylyltransferase (gtaB) CSB-YP758915SKY
CSB-EP758915SKY
CSB-BP758915SKY
CSB-MP758915SKY
CSB-EP758915SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Biotin synthase (bioB) CSB-YP758916SKY
CSB-EP758916SKY
CSB-BP758916SKY
CSB-MP758916SKY
CSB-EP758916SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative hemin transport system permease protein hrtB (hrtB), partial CSB-YP758917SKY1
CSB-EP758917SKY1
CSB-BP758917SKY1
CSB-MP758917SKY1
CSB-EP758917SKY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Urease accessory protein UreG (ureG) CSB-YP758918SKY
CSB-EP758918SKY
CSB-BP758918SKY
CSB-MP758918SKY
CSB-EP758918SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA topoisomerase 3 (topB), partial CSB-YP758919SKY
CSB-EP758919SKY
CSB-BP758919SKY
CSB-MP758919SKY
CSB-EP758919SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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