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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Prorocentrum minimum Ribulose bisphosphate carboxylase (rbcL) CSB-YP757867PZI
CSB-EP757867PZI
CSB-BP757867PZI
CSB-MP757867PZI
CSB-EP757867PZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine UDP-N-acetylglucosamine transporter (SLC35A3), partial CSB-YP757868BO
CSB-EP757868BO
CSB-BP757868BO
CSB-MP757868BO
CSB-EP757868BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vampyressa bidens Cytochrome b (MT-CYB), partial CSB-YP757869VAAF
CSB-EP757869VAAF
CSB-BP757869VAAF
CSB-MP757869VAAF
CSB-EP757869VAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brassica oleracea var. botrytis Xyloglucan endotransglucosylase/hydrolase (XET16A) CSB-YP757870BWF
CSB-EP757870BWF
CSB-BP757870BWF
CSB-MP757870BWF
CSB-EP757870BWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA replication and repair protein recF (recF) CSB-YP757871SIJ
CSB-EP757871SIJ
CSB-BP757871SIJ
CSB-MP757871SIJ
CSB-EP757871SIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glucuronoxylanase xynC (xynC) CSB-YP757872BRI
CSB-EP757872BRI
CSB-BP757872BRI
CSB-MP757872BRI
CSB-EP757872BRI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meloidogyne incognita CLAVATA3/ESR (CLE)-related protein 16D10 (16D10) CSB-YP757873MGAE
CSB-EP757873MGAE
CSB-BP757873MGAE
CSB-MP757873MGAE
CSB-EP757873MGAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dengue virus type 3 Genome polyprotein (pol), partial CSB-YP757874DCAQ
CSB-EP757874DCAQ
CSB-BP757874DCAQ
CSB-MP757874DCAQ
CSB-EP757874DCAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse G-protein coupled receptor 6 (Gpr6), partial CSB-YP757875MO1
CSB-EP757875MO1
CSB-BP757875MO1
CSB-MP757875MO1
CSB-EP757875MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picea engelmannii Maturase K (matK), partial CSB-YP757876PHAV
CSB-EP757876PHAV
CSB-BP757876PHAV
CSB-MP757876PHAV
CSB-EP757876PHAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Asparagine--tRNA ligase (asnS), partial CSB-YP757877OBN
CSB-EP757877OBN
CSB-BP757877OBN
CSB-MP757877OBN
CSB-EP757877OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 30S ribosomal protein S21 (rpsU) CSB-YP757878OBN
CSB-EP757878OBN
CSB-BP757878OBN
CSB-MP757878OBN
CSB-EP757878OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Phosphate acyltransferase (plsX) CSB-YP757879OBN
CSB-EP757879OBN
CSB-BP757879OBN
CSB-MP757879OBN
CSB-EP757879OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 30S ribosomal protein S4 (rpsD) CSB-YP757880OBN
CSB-EP757880OBN
CSB-BP757880OBN
CSB-MP757880OBN
CSB-EP757880OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Probable endonuclease 4 (nfo) CSB-YP757881OBN
CSB-EP757881OBN
CSB-BP757881OBN
CSB-MP757881OBN
CSB-EP757881OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma UvrABC system protein B (uvrB), partial CSB-YP757882OBN
CSB-EP757882OBN
CSB-BP757882OBN
CSB-MP757882OBN
CSB-EP757882OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma tRNA dimethylallyltransferase (miaA) CSB-YP757883OBN
CSB-EP757883OBN
CSB-BP757883OBN
CSB-MP757883OBN
CSB-EP757883OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Elongation factor Tu (tuf) CSB-YP757884OBN
CSB-EP757884OBN
CSB-BP757884OBN
CSB-MP757884OBN
CSB-EP757884OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 30S ribosomal protein S12 (rpsL) CSB-YP757885OBN
CSB-EP757885OBN
CSB-BP757885OBN
CSB-MP757885OBN
CSB-EP757885OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 50S ribosomal protein L36 (rpmJ) CSB-YP757886OBN
CSB-EP757886OBN
CSB-BP757886OBN
CSB-MP757886OBN
CSB-EP757886OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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