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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP757465CQF
CSB-EP757465CQF
CSB-BP757465CQF
CSB-MP757465CQF
CSB-EP757465CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-isopropylmalate dehydrogenase (leuB) CSB-YP757466CQF
CSB-EP757466CQF
CSB-BP757466CQF
CSB-MP757466CQF
CSB-EP757466CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA ligase (ligA), partial CSB-YP757467CQF
CSB-EP757467CQF
CSB-BP757467CQF
CSB-MP757467CQF
CSB-EP757467CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homoserine kinase (thrB) CSB-YP757468CQF
CSB-EP757468CQF
CSB-BP757468CQF
CSB-MP757468CQF
CSB-EP757468CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serine hydroxymethyltransferase (glyA) CSB-YP757469CQF
CSB-EP757469CQF
CSB-BP757469CQF
CSB-MP757469CQF
CSB-EP757469CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifunctional protein GlmU (glmU) CSB-YP757470CQF
CSB-EP757470CQF
CSB-BP757470CQF
CSB-MP757470CQF
CSB-EP757470CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S18 (rpsR) CSB-YP757471CQF
CSB-EP757471CQF
CSB-BP757471CQF
CSB-MP757471CQF
CSB-EP757471CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0182 protein DIP0733 (DIP0733), partial CSB-YP757472CQF
CSB-EP757472CQF
CSB-BP757472CQF
CSB-MP757472CQF
CSB-EP757472CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Error-prone DNA polymerase (dnaE2), partial CSB-YP757473CQF
CSB-EP757473CQF
CSB-BP757473CQF
CSB-MP757473CQF
CSB-EP757473CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP757474CQF
CSB-EP757474CQF
CSB-BP757474CQF
CSB-MP757474CQF
CSB-EP757474CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L30 (rpmD) CSB-YP757475CQF
CSB-EP757475CQF
CSB-BP757475CQF
CSB-MP757475CQF
CSB-EP757475CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S17 (rpsQ) CSB-YP757476CQF
CSB-EP757476CQF
CSB-BP757476CQF
CSB-MP757476CQF
CSB-EP757476CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S19 (rpsS) CSB-YP757477CQF
CSB-EP757477CQF
CSB-BP757477CQF
CSB-MP757477CQF
CSB-EP757477CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L7/L12 (rplL) CSB-YP757478CQF
CSB-EP757478CQF
CSB-BP757478CQF
CSB-MP757478CQF
CSB-EP757478CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Biotin synthase 1 (bioB1) CSB-YP757479CQF
CSB-EP757479CQF
CSB-BP757479CQF
CSB-MP757479CQF
CSB-EP757479CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At2g19280 (At2g19280), partial CSB-YP757480DOA
CSB-EP757480DOA
CSB-BP757480DOA
CSB-MP757480DOA
CSB-EP757480DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protection of telomeres protein 1b (POT1B) CSB-YP757481DOA
CSB-EP757481DOA
CSB-BP757481DOA
CSB-MP757481DOA
CSB-EP757481DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ABC transporter D family member 2, chloroplastic (ABCC2), partial CSB-YP757482DOA1
CSB-EP757482DOA1
CSB-BP757482DOA1
CSB-MP757482DOA1
CSB-EP757482DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cysteine-rich repeat secretory protein 3 (CRRSP3) CSB-YP757483DOA
CSB-EP757483DOA
CSB-BP757483DOA
CSB-MP757483DOA
CSB-EP757483DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Gibberellin-regulated protein 6 (GASA6) CSB-YP757484DOA
CSB-EP757484DOA
CSB-BP757484DOA
CSB-MP757484DOA
CSB-EP757484DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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