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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Photorhabdus luminescens subsp. laumondii Protein yebF (yebF) CSB-YP759413PIJ
CSB-EP759413PIJ
CSB-BP759413PIJ
CSB-MP759413PIJ
CSB-EP759413PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Vitamin B12 import ATP-binding protein BtuD (btuD) CSB-YP759414PIJ
CSB-EP759414PIJ
CSB-BP759414PIJ
CSB-MP759414PIJ
CSB-EP759414PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Hemin import ATP-binding protein HmuV (hmuV) CSB-YP759415PIJ
CSB-EP759415PIJ
CSB-BP759415PIJ
CSB-MP759415PIJ
CSB-EP759415PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP759416PIJ
CSB-EP759416PIJ
CSB-BP759416PIJ
CSB-MP759416PIJ
CSB-EP759416PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Fatty acid metabolism regulator protein (fadR) CSB-YP759417PIJ
CSB-EP759417PIJ
CSB-BP759417PIJ
CSB-MP759417PIJ
CSB-EP759417PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Probable intracellular septation protein A (plu2483), partial CSB-YP759418PIJ1
CSB-EP759418PIJ1
CSB-BP759418PIJ1
CSB-MP759418PIJ1
CSB-EP759418PIJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Fumarate hydratase class II (fumC) CSB-YP759419PIJ
CSB-EP759419PIJ
CSB-BP759419PIJ
CSB-MP759419PIJ
CSB-EP759419PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 3-phenylpropionate/cinnamic acid dioxygenase subunit alpha (hcaE) CSB-YP759420PIJ
CSB-EP759420PIJ
CSB-BP759420PIJ
CSB-MP759420PIJ
CSB-EP759420PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii FMN-dependent NADH-azoreductase (azoR) CSB-YP759421PIJ
CSB-EP759421PIJ
CSB-BP759421PIJ
CSB-MP759421PIJ
CSB-EP759421PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP759422PIJ
CSB-EP759422PIJ
CSB-BP759422PIJ
CSB-MP759422PIJ
CSB-EP759422PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Multidrug resistance protein MdtH (mdtH), partial CSB-YP759423PIJ
CSB-EP759423PIJ
CSB-BP759423PIJ
CSB-MP759423PIJ
CSB-EP759423PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Peptide chain release factor 1 (prfA) CSB-YP759424PIJ
CSB-EP759424PIJ
CSB-BP759424PIJ
CSB-MP759424PIJ
CSB-EP759424PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii UvrABC system protein C (uvrC), partial CSB-YP759425PIJ
CSB-EP759425PIJ
CSB-BP759425PIJ
CSB-MP759425PIJ
CSB-EP759425PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Sulfurtransferase TusE (tusE) CSB-YP759426PIJ
CSB-EP759426PIJ
CSB-BP759426PIJ
CSB-MP759426PIJ
CSB-EP759426PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Nicotinate phosphoribosyltransferase (pncB) CSB-YP759427PIJ
CSB-EP759427PIJ
CSB-BP759427PIJ
CSB-MP759427PIJ
CSB-EP759427PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Integration host factor subunit beta (ihfB) CSB-YP759428PIJ
CSB-EP759428PIJ
CSB-BP759428PIJ
CSB-MP759428PIJ
CSB-EP759428PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP759429PIJ
CSB-EP759429PIJ
CSB-BP759429PIJ
CSB-MP759429PIJ
CSB-EP759429PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP759430PIJ
CSB-EP759430PIJ
CSB-BP759430PIJ
CSB-MP759430PIJ
CSB-EP759430PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii UPF0299 membrane protein plu1549 (plu1549), partial CSB-YP759431PIJ1
CSB-EP759431PIJ1
CSB-BP759431PIJ1
CSB-MP759431PIJ1
CSB-EP759431PIJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Molybdenum import ATP-binding protein ModC (modC) CSB-YP759432PIJ
CSB-EP759432PIJ
CSB-BP759432PIJ
CSB-MP759432PIJ
CSB-EP759432PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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