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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Danio rerio Ribosome biogenesis protein wdr12 (wdr12) CSB-YP757525DIL
CSB-EP757525DIL
CSB-BP757525DIL
CSB-MP757525DIL
CSB-EP757525DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio UPF0461 protein C5orf24 homolog (zgc:77058) CSB-YP757526DIL
CSB-EP757526DIL
CSB-BP757526DIL
CSB-MP757526DIL
CSB-EP757526DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Vasculin (Gpbp1) CSB-YP757527MO
CSB-EP757527MO
CSB-BP757527MO
CSB-MP757527MO
CSB-EP757527MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Inactive dipeptidyl peptidase 10 (Dpp10), partial CSB-YP757528MO
CSB-EP757528MO
CSB-BP757528MO
CSB-MP757528MO
CSB-EP757528MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse WD repeat-containing protein 67 (Wdr67), partial CSB-YP757529MO
CSB-EP757529MO
CSB-BP757529MO
CSB-MP757529MO
CSB-EP757529MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Histone chaperone asf1b-B (asf1bb) CSB-YP757530DIL
CSB-EP757530DIL
CSB-BP757530DIL
CSB-MP757530DIL
CSB-EP757530DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Charged multivesicular body protein 3 (chmp3) CSB-YP757531DIL
CSB-EP757531DIL
CSB-BP757531DIL
CSB-MP757531DIL
CSB-EP757531DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Ras-related protein Rab-1A (Rab1A) CSB-YP757532RA
CSB-EP757532RA
CSB-BP757532RA
CSB-MP757532RA
CSB-EP757532RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Alkylglycerol monooxygenase (agmo), partial CSB-YP757533DIL1
CSB-EP757533DIL1
CSB-BP757533DIL1
CSB-MP757533DIL1
CSB-EP757533DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein YIPF4 (yipf4), partial CSB-YP757534DIL1
CSB-EP757534DIL1
CSB-BP757534DIL1
CSB-MP757534DIL1
CSB-EP757534DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio E3 ubiquitin-protein ligase MARCH5 (41338), partial CSB-YP757535DIL1
CSB-EP757535DIL1
CSB-BP757535DIL1
CSB-MP757535DIL1
CSB-EP757535DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Probable helicase with zinc finger domain (helz), partial CSB-YP757536DIL
CSB-EP757536DIL
CSB-BP757536DIL
CSB-MP757536DIL
CSB-EP757536DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio F-box/SPRY domain-containing protein 1 (fbxo45) CSB-YP757537DIL
CSB-EP757537DIL
CSB-BP757537DIL
CSB-MP757537DIL
CSB-EP757537DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Methyltransferase-like protein 14 (mettl14) CSB-YP757538DIL
CSB-EP757538DIL
CSB-BP757538DIL
CSB-MP757538DIL
CSB-EP757538DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Lissencephaly-1 homolog (pafah1b1) CSB-YP757539XBF
CSB-EP757539XBF
CSB-BP757539XBF
CSB-MP757539XBF
CSB-EP757539XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Uncharacterized protein C6orf174 homolog, partial CSB-YP757540MO
CSB-EP757540MO
CSB-BP757540MO
CSB-MP757540MO
CSB-EP757540MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse RNA-binding protein 26 (Rbm26), partial CSB-YP757541MO
CSB-EP757541MO
CSB-BP757541MO
CSB-MP757541MO
CSB-EP757541MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio UPF0669 protein C6orf120 homolog (zgc:77929) CSB-YP757542DIL
CSB-EP757542DIL
CSB-BP757542DIL
CSB-MP757542DIL
CSB-EP757542DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human F-box and leucine-rich protein 22 (FBXL22) CSB-YP757543HU
CSB-EP757543HU
CSB-BP757543HU
CSB-MP757543HU
CSB-EP757543HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ubiquitin-conjugating enzyme E2 J2 (Ube2j2), partial CSB-YP757544MO
CSB-EP757544MO
CSB-BP757544MO
CSB-MP757544MO
CSB-EP757544MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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