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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Photorhabdus luminescens subsp. laumondii Elongation factor G (fusA), partial CSB-YP759453PIJ
CSB-EP759453PIJ
CSB-BP759453PIJ
CSB-MP759453PIJ
CSB-EP759453PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii PTS system N-acetylmuramic acid-specific EIIBC component (murP), partial CSB-YP759454PIJ
CSB-EP759454PIJ
CSB-BP759454PIJ
CSB-MP759454PIJ
CSB-EP759454PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Der GTPase-activating protein YihI (yihI) CSB-YP759455PIJ
CSB-EP759455PIJ
CSB-BP759455PIJ
CSB-MP759455PIJ
CSB-EP759455PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Uronate isomerase (uxaC) CSB-YP759456PIJ
CSB-EP759456PIJ
CSB-BP759456PIJ
CSB-MP759456PIJ
CSB-EP759456PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 3-dehydroquinate synthase (aroB) CSB-YP759457PIJ
CSB-EP759457PIJ
CSB-BP759457PIJ
CSB-MP759457PIJ
CSB-EP759457PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Aspartate--ammonia ligase (asnA) CSB-YP759458PIJ
CSB-EP759458PIJ
CSB-BP759458PIJ
CSB-MP759458PIJ
CSB-EP759458PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii ATP synthase subunit beta (atpD) CSB-YP759459PIJ
CSB-EP759459PIJ
CSB-BP759459PIJ
CSB-MP759459PIJ
CSB-EP759459PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Serine--tRNA ligase (serS) CSB-YP759460MWI
CSB-EP759460MWI
CSB-BP759460MWI
CSB-MP759460MWI
CSB-EP759460MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Cysteine--tRNA ligase (cysS) CSB-YP759461MWI
CSB-EP759461MWI
CSB-BP759461MWI
CSB-MP759461MWI
CSB-EP759461MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum 50S ribosomal protein L9 (rplI) CSB-YP759462MWI
CSB-EP759462MWI
CSB-BP759462MWI
CSB-MP759462MWI
CSB-EP759462MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum tRNA pseudouridine synthase A (truA) CSB-YP759463MWI
CSB-EP759463MWI
CSB-BP759463MWI
CSB-MP759463MWI
CSB-EP759463MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Tryptophan--tRNA ligase (trpS) CSB-YP759464MWI
CSB-EP759464MWI
CSB-BP759464MWI
CSB-MP759464MWI
CSB-EP759464MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Elongation factor P (efp) CSB-YP759465MWI
CSB-EP759465MWI
CSB-BP759465MWI
CSB-MP759465MWI
CSB-EP759465MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP759466MWI
CSB-EP759466MWI
CSB-BP759466MWI
CSB-MP759466MWI
CSB-EP759466MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Protein MraZ (mraZ) CSB-YP759467MWI
CSB-EP759467MWI
CSB-BP759467MWI
CSB-MP759467MWI
CSB-EP759467MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum 50S ribosomal protein L27 (rpmA) CSB-YP759468MWI
CSB-EP759468MWI
CSB-BP759468MWI
CSB-MP759468MWI
CSB-EP759468MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Heat-inducible transcription repressor HrcA (hrcA) CSB-YP759469MWI
CSB-EP759469MWI
CSB-BP759469MWI
CSB-MP759469MWI
CSB-EP759469MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Phosphoglycerate kinase (pgk) CSB-YP759470MWI
CSB-EP759470MWI
CSB-BP759470MWI
CSB-MP759470MWI
CSB-EP759470MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Transcription elongation factor GreA (greA) CSB-YP759471MWI
CSB-EP759471MWI
CSB-BP759471MWI
CSB-MP759471MWI
CSB-EP759471MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Cytidylate kinase (cmk) CSB-YP759472MWI
CSB-EP759472MWI
CSB-BP759472MWI
CSB-MP759472MWI
CSB-EP759472MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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