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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Photorhabdus luminescens subsp. laumondii Potassium-transporting ATPase C chain (kdpC), partial CSB-YP759433PIJ
CSB-EP759433PIJ
CSB-BP759433PIJ
CSB-MP759433PIJ
CSB-EP759433PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Histidine--tRNA ligase (hisS) CSB-YP759434PIJ
CSB-EP759434PIJ
CSB-BP759434PIJ
CSB-MP759434PIJ
CSB-EP759434PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Leucine--tRNA ligase (leuS), partial CSB-YP759435PIJ
CSB-EP759435PIJ
CSB-BP759435PIJ
CSB-MP759435PIJ
CSB-EP759435PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 50S ribosomal protein L19 (rplS) CSB-YP759436PIJ
CSB-EP759436PIJ
CSB-BP759436PIJ
CSB-MP759436PIJ
CSB-EP759436PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Bifunctional protein aas (aas), partial CSB-YP759437PIJ1
CSB-EP759437PIJ1
CSB-BP759437PIJ1
CSB-MP759437PIJ1
CSB-EP759437PIJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Na (+)-translocating NADH-quinone reductase subunit E (nqrE), partial CSB-YP759438PIJ1
CSB-EP759438PIJ1
CSB-BP759438PIJ1
CSB-MP759438PIJ1
CSB-EP759438PIJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Probable Fe (2+)-trafficking protein (plu1168) CSB-YP759439PIJ
CSB-EP759439PIJ
CSB-BP759439PIJ
CSB-MP759439PIJ
CSB-EP759439PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Glutamyl-Q tRNA (Asp) synthetase (gluQ) CSB-YP759440PIJ
CSB-EP759440PIJ
CSB-BP759440PIJ
CSB-MP759440PIJ
CSB-EP759440PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Tryptophanase (tnaA) CSB-YP759441PIJ
CSB-EP759441PIJ
CSB-BP759441PIJ
CSB-MP759441PIJ
CSB-EP759441PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP759442PIJ
CSB-EP759442PIJ
CSB-BP759442PIJ
CSB-MP759442PIJ
CSB-EP759442PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP759443PIJ
CSB-EP759443PIJ
CSB-BP759443PIJ
CSB-MP759443PIJ
CSB-EP759443PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Ribonuclease HII (rnhB) CSB-YP759444PIJ
CSB-EP759444PIJ
CSB-BP759444PIJ
CSB-MP759444PIJ
CSB-EP759444PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Ribosome-recycling factor (frr) CSB-YP759445PIJ
CSB-EP759445PIJ
CSB-BP759445PIJ
CSB-MP759445PIJ
CSB-EP759445PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Protein Xni (xni) CSB-YP759446PIJ
CSB-EP759446PIJ
CSB-BP759446PIJ
CSB-MP759446PIJ
CSB-EP759446PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Thiamine import ATP-binding protein ThiQ (thiQ) CSB-YP759447PIJ
CSB-EP759447PIJ
CSB-BP759447PIJ
CSB-MP759447PIJ
CSB-EP759447PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Dihydrodipicolinate reductase (dapB) CSB-YP759448PIJ
CSB-EP759448PIJ
CSB-BP759448PIJ
CSB-MP759448PIJ
CSB-EP759448PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Na (+)/H (+) antiporter nhaA (nhaA), partial CSB-YP759449PIJ
CSB-EP759449PIJ
CSB-BP759449PIJ
CSB-MP759449PIJ
CSB-EP759449PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Phosphopentomutase (deoB) CSB-YP759450PIJ
CSB-EP759450PIJ
CSB-BP759450PIJ
CSB-MP759450PIJ
CSB-EP759450PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Thiamine-phosphate synthase (thiE) CSB-YP759451PIJ
CSB-EP759451PIJ
CSB-BP759451PIJ
CSB-MP759451PIJ
CSB-EP759451PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Maltose/maltodextrin import ATP-binding protein MalK (malK) CSB-YP759452PIJ
CSB-EP759452PIJ
CSB-BP759452PIJ
CSB-MP759452PIJ
CSB-EP759452PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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