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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Gloeobacter violaceus NAD (P)H-quinone oxidoreductase subunit M (ndhM) CSB-YP759513GCI
CSB-EP759513GCI
CSB-BP759513GCI
CSB-MP759513GCI
CSB-EP759513GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus N utilization substance protein B homolog (nusB) CSB-YP759514GCI
CSB-EP759514GCI
CSB-BP759514GCI
CSB-MP759514GCI
CSB-EP759514GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus 15,16-dihydrobiliverdin:ferredoxin oxidoreductase (pebA) CSB-YP759515GCI
CSB-EP759515GCI
CSB-BP759515GCI
CSB-MP759515GCI
CSB-EP759515GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Sulfate adenylyltransferase (sat) CSB-YP759516GCI
CSB-EP759516GCI
CSB-BP759516GCI
CSB-MP759516GCI
CSB-EP759516GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Lipoyl synthase 1 (lipA1) CSB-YP759517GCI
CSB-EP759517GCI
CSB-BP759517GCI
CSB-MP759517GCI
CSB-EP759517GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Ribose-phosphate pyrophosphokinase (prs) CSB-YP759518GCI
CSB-EP759518GCI
CSB-BP759518GCI
CSB-MP759518GCI
CSB-EP759518GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Phosphopantetheine adenylyltransferase (coaD) CSB-YP759519GCI
CSB-EP759519GCI
CSB-BP759519GCI
CSB-MP759519GCI
CSB-EP759519GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus 50S ribosomal protein L32 (rpmF) CSB-YP759520GCI
CSB-EP759520GCI
CSB-BP759520GCI
CSB-MP759520GCI
CSB-EP759520GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus NAD (P)H-quinone oxidoreductase subunit K 1 (ndhK1) CSB-YP759521GCI
CSB-EP759521GCI
CSB-BP759521GCI
CSB-MP759521GCI
CSB-EP759521GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP759522GCI
CSB-EP759522GCI
CSB-BP759522GCI
CSB-MP759522GCI
CSB-EP759522GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Potassium-transporting ATPase A chain (kdpA), partial CSB-YP759523GCI
CSB-EP759523GCI
CSB-BP759523GCI
CSB-MP759523GCI
CSB-EP759523GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Phosphate import ATP-binding protein PstB 2 (pstB2) CSB-YP759524GCI
CSB-EP759524GCI
CSB-BP759524GCI
CSB-MP759524GCI
CSB-EP759524GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus 50S ribosomal protein L25 (rplY) CSB-YP759525GCI
CSB-EP759525GCI
CSB-BP759525GCI
CSB-MP759525GCI
CSB-EP759525GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP759526GCI
CSB-EP759526GCI
CSB-BP759526GCI
CSB-MP759526GCI
CSB-EP759526GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus DNA repair protein recO (recO) CSB-YP759527GCI
CSB-EP759527GCI
CSB-BP759527GCI
CSB-MP759527GCI
CSB-EP759527GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Ribose-5-phosphate isomerase A (rpiA) CSB-YP759528GCI
CSB-EP759528GCI
CSB-BP759528GCI
CSB-MP759528GCI
CSB-EP759528GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum tRNA modification GTPase MnmE (mnmE) CSB-YP759529CKA
CSB-EP759529CKA
CSB-BP759529CKA
CSB-MP759529CKA
CSB-EP759529CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP759530CKA
CSB-EP759530CKA
CSB-BP759530CKA
CSB-MP759530CKA
CSB-EP759530CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Protein translocase subunit SecA (secA), partial CSB-YP759531CKA
CSB-EP759531CKA
CSB-BP759531CKA
CSB-MP759531CKA
CSB-EP759531CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Orotate phosphoribosyltransferase (pyrE) CSB-YP759532CKA
CSB-EP759532CKA
CSB-BP759532CKA
CSB-MP759532CKA
CSB-EP759532CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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