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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chromobacterium violaceum 50S ribosomal protein L1 (rplA) CSB-YP759533CKA
CSB-EP759533CKA
CSB-BP759533CKA
CSB-MP759533CKA
CSB-EP759533CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Elongation factor G (fusA), partial CSB-YP759534CKA
CSB-EP759534CKA
CSB-BP759534CKA
CSB-MP759534CKA
CSB-EP759534CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 30S ribosomal protein S17 (rpsQ) CSB-YP759535CKA
CSB-EP759535CKA
CSB-BP759535CKA
CSB-MP759535CKA
CSB-EP759535CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 50S ribosomal protein L30 (rpmD) CSB-YP759536CKA
CSB-EP759536CKA
CSB-BP759536CKA
CSB-MP759536CKA
CSB-EP759536CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Spermidine/putrescine import ATP-binding protein PotA (potA) CSB-YP759537CKA
CSB-EP759537CKA
CSB-BP759537CKA
CSB-MP759537CKA
CSB-EP759537CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Protoheme IX farnesyltransferase 2 (ctaB2), partial CSB-YP759538CKA
CSB-EP759538CKA
CSB-BP759538CKA
CSB-MP759538CKA
CSB-EP759538CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum tRNA (guanine-N (7)-)-methyltransferase (trmB) CSB-YP759539CKA
CSB-EP759539CKA
CSB-BP759539CKA
CSB-MP759539CKA
CSB-EP759539CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Thiazole synthase (thiG) CSB-YP759540CKA
CSB-EP759540CKA
CSB-BP759540CKA
CSB-MP759540CKA
CSB-EP759540CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum C4-dicarboxylate transport protein (dctA), partial CSB-YP759541CKA
CSB-EP759541CKA
CSB-BP759541CKA
CSB-MP759541CKA
CSB-EP759541CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 30S ribosomal protein S16 (rpsP) CSB-YP759542CKA
CSB-EP759542CKA
CSB-BP759542CKA
CSB-MP759542CKA
CSB-EP759542CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 50S ribosomal protein L9 (rplI) CSB-YP759543CKA
CSB-EP759543CKA
CSB-BP759543CKA
CSB-MP759543CKA
CSB-EP759543CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Isoleucine--tRNA ligase (ileS), partial CSB-YP759544CKA
CSB-EP759544CKA
CSB-BP759544CKA
CSB-MP759544CKA
CSB-EP759544CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Histidine--tRNA ligase (hisS) CSB-YP759545CKA
CSB-EP759545CKA
CSB-BP759545CKA
CSB-MP759545CKA
CSB-EP759545CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 7-cyano-7-deazaguanine synthase (queC) CSB-YP759546CKA
CSB-EP759546CKA
CSB-BP759546CKA
CSB-MP759546CKA
CSB-EP759546CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 50S ribosomal protein L28 (rpmB) CSB-YP759547CKA
CSB-EP759547CKA
CSB-BP759547CKA
CSB-MP759547CKA
CSB-EP759547CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Nicotinate phosphoribosyltransferase (pncB) CSB-YP759548CKA
CSB-EP759548CKA
CSB-BP759548CKA
CSB-MP759548CKA
CSB-EP759548CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (coq7) CSB-YP759549CKA
CSB-EP759549CKA
CSB-BP759549CKA
CSB-MP759549CKA
CSB-EP759549CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum UPF0042 nucleotide-binding protein CV_3336 (CV_3336) CSB-YP759550CKA
CSB-EP759550CKA
CSB-BP759550CKA
CSB-MP759550CKA
CSB-EP759550CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP759551CKA
CSB-EP759551CKA
CSB-BP759551CKA
CSB-MP759551CKA
CSB-EP759551CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum UPF0250 protein CV_3095 (CV_3095) CSB-YP759552CKA
CSB-EP759552CKA
CSB-BP759552CKA
CSB-MP759552CKA
CSB-EP759552CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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