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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chromobacterium violaceum Ribose import ATP-binding protein RbsA (rbsA), partial CSB-YP759553CKA
CSB-EP759553CKA
CSB-BP759553CKA
CSB-MP759553CKA
CSB-EP759553CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Flagellar L-ring protein 2 (flgH2) CSB-YP759554CKA
CSB-EP759554CKA
CSB-BP759554CKA
CSB-MP759554CKA
CSB-EP759554CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 3-isopropylmalate dehydrogenase (leuB) CSB-YP759555CKA
CSB-EP759555CKA
CSB-BP759555CKA
CSB-MP759555CKA
CSB-EP759555CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Probable potassium transport system protein kup 1 (kup1), partial CSB-YP759556CKA
CSB-EP759556CKA
CSB-BP759556CKA
CSB-MP759556CKA
CSB-EP759556CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP759557CKA
CSB-EP759557CKA
CSB-BP759557CKA
CSB-MP759557CKA
CSB-EP759557CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Probable RNA 2'-phosphotransferase (kptA) CSB-YP759558CKA
CSB-EP759558CKA
CSB-BP759558CKA
CSB-MP759558CKA
CSB-EP759558CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Putative pterin-4-alpha-carbinolamine dehydratase (CV_2366) CSB-YP759559CKA
CSB-EP759559CKA
CSB-BP759559CKA
CSB-MP759559CKA
CSB-EP759559CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Lipid-A-disaccharide synthase (lpxB) CSB-YP759560CKA
CSB-EP759560CKA
CSB-BP759560CKA
CSB-MP759560CKA
CSB-EP759560CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Protein ApaG (apaG) CSB-YP759561CKA
CSB-EP759561CKA
CSB-BP759561CKA
CSB-MP759561CKA
CSB-EP759561CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum DNA repair protein recO (recO) CSB-YP759562CKA
CSB-EP759562CKA
CSB-BP759562CKA
CSB-MP759562CKA
CSB-EP759562CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Undecaprenyl-diphosphatase 2 (uppP2), partial CSB-YP759563CKA
CSB-EP759563CKA
CSB-BP759563CKA
CSB-MP759563CKA
CSB-EP759563CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum UPF0597 protein CV_1824 (CV_1824) CSB-YP759564CKA
CSB-EP759564CKA
CSB-BP759564CKA
CSB-MP759564CKA
CSB-EP759564CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Glutamine--tRNA ligase (glnS), partial CSB-YP759565CKA
CSB-EP759565CKA
CSB-BP759565CKA
CSB-MP759565CKA
CSB-EP759565CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Chaperone protein DnaK (dnaK), partial CSB-YP759566CKA
CSB-EP759566CKA
CSB-BP759566CKA
CSB-MP759566CKA
CSB-EP759566CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Protein RecA (recA) CSB-YP759567CKA
CSB-EP759567CKA
CSB-BP759567CKA
CSB-MP759567CKA
CSB-EP759567CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Cobalamin biosynthesis protein CobD (cobD), partial CSB-YP759568CKA1
CSB-EP759568CKA1
CSB-BP759568CKA1
CSB-MP759568CKA1
CSB-EP759568CKA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Translation initiation factor IF-2 (infB), partial CSB-YP759569CKA
CSB-EP759569CKA
CSB-BP759569CKA
CSB-MP759569CKA
CSB-EP759569CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Integration host factor subunit alpha (ihfA) CSB-YP759570CKA
CSB-EP759570CKA
CSB-BP759570CKA
CSB-MP759570CKA
CSB-EP759570CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Undecaprenyl-diphosphatase 1 (uppP1), partial CSB-YP759571CKA1
CSB-EP759571CKA1
CSB-BP759571CKA1
CSB-MP759571CKA1
CSB-EP759571CKA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Ribose-5-phosphate isomerase A (rpiA) CSB-YP759572CKA
CSB-EP759572CKA
CSB-BP759572CKA
CSB-MP759572CKA
CSB-EP759572CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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