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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Prochlorococcus marinus Polyphosphate kinase (ppk), partial CSB-YP759933EYO
CSB-EP759933EYO
CSB-BP759933EYO
CSB-MP759933EYO
CSB-EP759933EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Nucleoside diphosphate kinase (ndk) CSB-YP759934EYO
CSB-EP759934EYO
CSB-BP759934EYO
CSB-MP759934EYO
CSB-EP759934EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L1 (rplA) CSB-YP759935EYO
CSB-EP759935EYO
CSB-BP759935EYO
CSB-MP759935EYO
CSB-EP759935EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit 1 (ndhA), partial CSB-YP759936EYO
CSB-EP759936EYO
CSB-BP759936EYO
CSB-MP759936EYO
CSB-EP759936EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus UPF0367 protein PMT_1986 (PMT_1986) CSB-YP759937EYO
CSB-EP759937EYO
CSB-BP759937EYO
CSB-MP759937EYO
CSB-EP759937EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Histidine--tRNA ligase (hisS) CSB-YP759938EYO
CSB-EP759938EYO
CSB-BP759938EYO
CSB-MP759938EYO
CSB-EP759938EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP759939EYO
CSB-EP759939EYO
CSB-BP759939EYO
CSB-MP759939EYO
CSB-EP759939EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Serine hydroxymethyltransferase (glyA) CSB-YP759940EYO
CSB-EP759940EYO
CSB-BP759940EYO
CSB-MP759940EYO
CSB-EP759940EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus tRNA (guanine-N (7)-)-methyltransferase (trmB) CSB-YP759941EYO
CSB-EP759941EYO
CSB-BP759941EYO
CSB-MP759941EYO
CSB-EP759941EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S7 (rpsG) CSB-YP759942EYO
CSB-EP759942EYO
CSB-BP759942EYO
CSB-MP759942EYO
CSB-EP759942EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L13 (rplM) CSB-YP759943EYO
CSB-EP759943EYO
CSB-BP759943EYO
CSB-MP759943EYO
CSB-EP759943EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L18 (rplR) CSB-YP759944EYO
CSB-EP759944EYO
CSB-BP759944EYO
CSB-MP759944EYO
CSB-EP759944EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L22 (rplV) CSB-YP759945EYO
CSB-EP759945EYO
CSB-BP759945EYO
CSB-MP759945EYO
CSB-EP759945EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus DNA replication and repair protein recF (recF) CSB-YP759946EYO
CSB-EP759946EYO
CSB-BP759946EYO
CSB-MP759946EYO
CSB-EP759946EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus S-adenosylmethionine synthase (metK) CSB-YP759947EYO
CSB-EP759947EYO
CSB-BP759947EYO
CSB-MP759947EYO
CSB-EP759947EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ribose-5-phosphate isomerase A (rpiA) CSB-YP759948EYO
CSB-EP759948EYO
CSB-BP759948EYO
CSB-MP759948EYO
CSB-EP759948EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP759949EYO
CSB-EP759949EYO
CSB-BP759949EYO
CSB-MP759949EYO
CSB-EP759949EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus ATP synthase subunit b (atpF), partial CSB-YP759950EYO1
CSB-EP759950EYO1
CSB-BP759950EYO1
CSB-MP759950EYO1
CSB-EP759950EYO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cytochrome c-550 (psbV) CSB-YP759951EYO
CSB-EP759951EYO
CSB-BP759951EYO
CSB-MP759951EYO
CSB-EP759951EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP759952EYO
CSB-EP759952EYO
CSB-BP759952EYO
CSB-MP759952EYO
CSB-EP759952EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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