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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus Ribonuclease P protein component (rnpA) CSB-YP759953EYO
CSB-EP759953EYO
CSB-BP759953EYO
CSB-MP759953EYO
CSB-EP759953EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem I reaction center subunit IX (psaJ), partial CSB-YP759954EYO
CSB-EP759954EYO
CSB-BP759954EYO
CSB-MP759954EYO
CSB-EP759954EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Transaldolase (tal) CSB-YP759955EYO
CSB-EP759955EYO
CSB-BP759955EYO
CSB-MP759955EYO
CSB-EP759955EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Light-independent protochlorophyllide reductase subunit N (chlN) CSB-YP759956EYO
CSB-EP759956EYO
CSB-BP759956EYO
CSB-MP759956EYO
CSB-EP759956EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem I assembly protein Ycf4 (ycf4), partial CSB-YP759957EYO1
CSB-EP759957EYO1
CSB-BP759957EYO1
CSB-MP759957EYO1
CSB-EP759957EYO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Recombination protein RecR (recR) CSB-YP759958EYO
CSB-EP759958EYO
CSB-BP759958EYO
CSB-MP759958EYO
CSB-EP759958EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP759959EYO
CSB-EP759959EYO
CSB-BP759959EYO
CSB-MP759959EYO
CSB-EP759959EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Chaperone protein dnaK1 (dnaK1), partial CSB-YP759960EYO
CSB-EP759960EYO
CSB-BP759960EYO
CSB-MP759960EYO
CSB-EP759960EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Glutamyl-tRNA (Gln) amidotransferase subunit A (gatA) CSB-YP759961EYO
CSB-EP759961EYO
CSB-BP759961EYO
CSB-MP759961EYO
CSB-EP759961EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cytochrome c biogenesis protein CcsA (ccsA), partial CSB-YP759962EYO
CSB-EP759962EYO
CSB-BP759962EYO
CSB-MP759962EYO
CSB-EP759962EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Putative Holliday junction resolvase (PMT_0526) CSB-YP759963EYO
CSB-EP759963EYO
CSB-BP759963EYO
CSB-MP759963EYO
CSB-EP759963EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Threonine--tRNA ligase (thrS), partial CSB-YP759964EYO
CSB-EP759964EYO
CSB-BP759964EYO
CSB-MP759964EYO
CSB-EP759964EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus ATP-dependent Clp protease proteolytic subunit 2 (clpP2) CSB-YP759965EYO
CSB-EP759965EYO
CSB-BP759965EYO
CSB-MP759965EYO
CSB-EP759965EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP759966EYO
CSB-EP759966EYO
CSB-BP759966EYO
CSB-MP759966EYO
CSB-EP759966EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP759967EYO
CSB-EP759967EYO
CSB-BP759967EYO
CSB-MP759967EYO
CSB-EP759967EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Adenosylhomocysteinase (ahcY) CSB-YP759968EYO
CSB-EP759968EYO
CSB-BP759968EYO
CSB-MP759968EYO
CSB-EP759968EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus ATP-dependent Clp protease proteolytic subunit 1 (clpP1) CSB-YP759969EYO
CSB-EP759969EYO
CSB-BP759969EYO
CSB-MP759969EYO
CSB-EP759969EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Elongation factor P (efp) CSB-YP759970EYO
CSB-EP759970EYO
CSB-BP759970EYO
CSB-MP759970EYO
CSB-EP759970EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP759971EYP
CSB-EP759971EYP
CSB-BP759971EYP
CSB-MP759971EYP
CSB-EP759971EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Glycine dehydrogenase [decarboxylating] (gcvP), partial CSB-YP759972EYP
CSB-EP759972EYP
CSB-BP759972EYP
CSB-MP759972EYP
CSB-EP759972EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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