Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Prochlorococcus marinus subsp. pastoris Dihydrodipicolinate reductase (dapB) CSB-YP759913EYQ
CSB-EP759913EYQ
CSB-BP759913EYQ
CSB-MP759913EYQ
CSB-EP759913EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris D-fructose 1,6-bisphosphatase class 2/sedoheptulose 1,7-bisphosphatase (PMM0767) CSB-YP759914EYQ
CSB-EP759914EYQ
CSB-BP759914EYQ
CSB-MP759914EYQ
CSB-EP759914EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 33 kDa chaperonin (hslO) CSB-YP759915EYQ
CSB-EP759915EYQ
CSB-BP759915EYQ
CSB-MP759915EYQ
CSB-EP759915EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Homoserine kinase (thrB) CSB-YP759916EYQ
CSB-EP759916EYQ
CSB-BP759916EYQ
CSB-MP759916EYQ
CSB-EP759916EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Chaperone protein ClpB (clpB), partial CSB-YP759917EYQ
CSB-EP759917EYQ
CSB-BP759917EYQ
CSB-MP759917EYQ
CSB-EP759917EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Ferrochelatase (hemH) CSB-YP759918EYQ
CSB-EP759918EYQ
CSB-BP759918EYQ
CSB-MP759918EYQ
CSB-EP759918EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Putative pterin-4-alpha-carbinolamine dehydratase (PMM0491) CSB-YP759919EYQ
CSB-EP759919EYQ
CSB-BP759919EYQ
CSB-MP759919EYQ
CSB-EP759919EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Cytochrome b6-f complex iron-sulfur subunit (petC), partial CSB-YP759920EYQ1
CSB-EP759920EYQ1
CSB-BP759920EYQ1
CSB-MP759920EYQ1
CSB-EP759920EYQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Elongation factor 4 (lepA), partial CSB-YP759921EYQ
CSB-EP759921EYQ
CSB-BP759921EYQ
CSB-MP759921EYQ
CSB-EP759921EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Cell division protein SepF (sepF) CSB-YP759922EYQ
CSB-EP759922EYQ
CSB-BP759922EYQ
CSB-MP759922EYQ
CSB-EP759922EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Cell division topological specificity factor (minE) CSB-YP759923EYQ
CSB-EP759923EYQ
CSB-BP759923EYQ
CSB-MP759923EYQ
CSB-EP759923EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Cytochrome b559 subunit beta (psbF), partial CSB-YP759924EYQ
CSB-EP759924EYQ
CSB-BP759924EYQ
CSB-MP759924EYQ
CSB-EP759924EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP759925EYQ
CSB-EP759925EYQ
CSB-BP759925EYQ
CSB-MP759925EYQ
CSB-EP759925EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Phosphoglucosamine mutase (glmM) CSB-YP759926EYQ
CSB-EP759926EYQ
CSB-BP759926EYQ
CSB-MP759926EYQ
CSB-EP759926EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Enolase (eno) CSB-YP759927EYQ
CSB-EP759927EYQ
CSB-BP759927EYQ
CSB-MP759927EYQ
CSB-EP759927EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Arginine--tRNA ligase (argS), partial CSB-YP759928EYQ
CSB-EP759928EYQ
CSB-BP759928EYQ
CSB-MP759928EYQ
CSB-EP759928EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris NAD (P)H-quinone oxidoreductase subunit M (ndhM) CSB-YP759929EYQ
CSB-EP759929EYQ
CSB-BP759929EYQ
CSB-MP759929EYQ
CSB-EP759929EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP759930EYQ
CSB-EP759930EYQ
CSB-BP759930EYQ
CSB-MP759930EYQ
CSB-EP759930EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Alanine--tRNA ligase (alaS), partial CSB-YP759931EYQ
CSB-EP759931EYQ
CSB-BP759931EYQ
CSB-MP759931EYQ
CSB-EP759931EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Protein grpE (grpE) CSB-YP759932EYQ
CSB-EP759932EYQ
CSB-BP759932EYQ
CSB-MP759932EYQ
CSB-EP759932EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>