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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus DNA mismatch repair protein MutS (mutS), partial CSB-YP759973EYP
CSB-EP759973EYP
CSB-BP759973EYP
CSB-MP759973EYP
CSB-EP759973EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP759974EYP
CSB-EP759974EYP
CSB-BP759974EYP
CSB-MP759974EYP
CSB-EP759974EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP759975EYP
CSB-EP759975EYP
CSB-BP759975EYP
CSB-MP759975EYP
CSB-EP759975EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L2 (rplB) CSB-YP759976EYP
CSB-EP759976EYP
CSB-BP759976EYP
CSB-MP759976EYP
CSB-EP759976EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L14 (rplN) CSB-YP759977EYP
CSB-EP759977EYP
CSB-BP759977EYP
CSB-MP759977EYP
CSB-EP759977EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L15 (rplO) CSB-YP759978EYP
CSB-EP759978EYP
CSB-BP759978EYP
CSB-MP759978EYP
CSB-EP759978EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S12 (rpsL) CSB-YP759979EYP
CSB-EP759979EYP
CSB-BP759979EYP
CSB-MP759979EYP
CSB-EP759979EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus LL-diaminopimelate aminotransferase (dapL) CSB-YP759980EYP
CSB-EP759980EYP
CSB-BP759980EYP
CSB-MP759980EYP
CSB-EP759980EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP759981EYP
CSB-EP759981EYP
CSB-BP759981EYP
CSB-MP759981EYP
CSB-EP759981EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus ATP synthase subunit b (atpF), partial CSB-YP759982EYP1
CSB-EP759982EYP1
CSB-BP759982EYP1
CSB-MP759982EYP1
CSB-EP759982EYP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP759983EYP
CSB-EP759983EYP
CSB-BP759983EYP
CSB-MP759983EYP
CSB-EP759983EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L27 (rpmA) CSB-YP759984EYP
CSB-EP759984EYP
CSB-BP759984EYP
CSB-MP759984EYP
CSB-EP759984EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ketol-acid reductoisomerase (ilvC) CSB-YP759985EYP
CSB-EP759985EYP
CSB-BP759985EYP
CSB-MP759985EYP
CSB-EP759985EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP759986EYP
CSB-EP759986EYP
CSB-BP759986EYP
CSB-MP759986EYP
CSB-EP759986EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S14 (rpsN) CSB-YP759987EYP
CSB-EP759987EYP
CSB-BP759987EYP
CSB-MP759987EYP
CSB-EP759987EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem I assembly protein Ycf4 (ycf4), partial CSB-YP759988EYP1
CSB-EP759988EYP1
CSB-BP759988EYP1
CSB-MP759988EYP1
CSB-EP759988EYP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cytochrome b6-f complex subunit 5 (petG), partial CSB-YP759989EYP
CSB-EP759989EYP
CSB-BP759989EYP
CSB-MP759989EYP
CSB-EP759989EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ribose-phosphate pyrophosphokinase (prs) CSB-YP759990EYP
CSB-EP759990EYP
CSB-BP759990EYP
CSB-MP759990EYP
CSB-EP759990EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP759991EYP
CSB-EP759991EYP
CSB-BP759991EYP
CSB-MP759991EYP
CSB-EP759991EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 33 kDa chaperonin (hslO) CSB-YP759992EYP
CSB-EP759992EYP
CSB-BP759992EYP
CSB-MP759992EYP
CSB-EP759992EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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