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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant 30S ribosomal protein S17 (rpsQ) CSB-YP762036MLA
CSB-EP762036MLA
CSB-BP762036MLA
CSB-MP762036MLA
CSB-EP762036MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L3 (rplC) CSB-YP762037MLA
CSB-EP762037MLA
CSB-BP762037MLA
CSB-MP762037MLA
CSB-EP762037MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP762038MLA
CSB-EP762038MLA
CSB-BP762038MLA
CSB-MP762038MLA
CSB-EP762038MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative S-adenosyl-L-methionine-dependent methyltransferase MAP_4078 (MAP_4078) CSB-YP762039MLA
CSB-EP762039MLA
CSB-BP762039MLA
CSB-MP762039MLA
CSB-EP762039MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP762040MLA
CSB-EP762040MLA
CSB-BP762040MLA
CSB-MP762040MLA
CSB-EP762040MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP762041MLA
CSB-EP762041MLA
CSB-BP762041MLA
CSB-MP762041MLA
CSB-EP762041MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein grpE (grpE) CSB-YP762042MLA
CSB-EP762042MLA
CSB-BP762042MLA
CSB-MP762042MLA
CSB-EP762042MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant R2-like ligand binding oxidase (MAP_3672) CSB-YP762043MLA
CSB-EP762043MLA
CSB-BP762043MLA
CSB-MP762043MLA
CSB-EP762043MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maltokinase (pep2) CSB-YP762044MLA
CSB-EP762044MLA
CSB-BP762044MLA
CSB-MP762044MLA
CSB-EP762044MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0353 protein MAP_3435c (MAP_3435c), partial CSB-YP762045MLA1
CSB-EP762045MLA1
CSB-BP762045MLA1
CSB-MP762045MLA1
CSB-EP762045MLA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipoprotein lpqB (lpqB), partial CSB-YP762046MLA
CSB-EP762046MLA
CSB-BP762046MLA
CSB-MP762046MLA
CSB-EP762046MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP762047MLA
CSB-EP762047MLA
CSB-BP762047MLA
CSB-MP762047MLA
CSB-EP762047MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable cytochrome c oxidase subunit 1 (ctaD), partial CSB-YP762048MLA
CSB-EP762048MLA
CSB-BP762048MLA
CSB-MP762048MLA
CSB-EP762048MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP762049MLA
CSB-EP762049MLA
CSB-BP762049MLA
CSB-MP762049MLA
CSB-EP762049MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP762050MLA
CSB-EP762050MLA
CSB-BP762050MLA
CSB-MP762050MLA
CSB-EP762050MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uridylate kinase (pyrH) CSB-YP762051MLA
CSB-EP762051MLA
CSB-BP762051MLA
CSB-MP762051MLA
CSB-EP762051MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Translation initiation factor IF-2 (infB), partial CSB-YP762052MLA
CSB-EP762052MLA
CSB-BP762052MLA
CSB-MP762052MLA
CSB-EP762052MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydrodipicolinate synthase (dapA) CSB-YP762053MLA
CSB-EP762053MLA
CSB-BP762053MLA
CSB-MP762053MLA
CSB-EP762053MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Threonine--tRNA ligase (thrS), partial CSB-YP762054MLA
CSB-EP762054MLA
CSB-BP762054MLA
CSB-MP762054MLA
CSB-EP762054MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP762055MLA
CSB-EP762055MLA
CSB-BP762055MLA
CSB-MP762055MLA
CSB-EP762055MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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