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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Geobacter sulfurreducens Putative phosphotransferase GSU0450 (GSU0450) CSB-YP762116GBK
CSB-EP762116GBK
CSB-BP762116GBK
CSB-MP762116GBK
CSB-EP762116GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP762117GBK
CSB-EP762117GBK
CSB-BP762117GBK
CSB-MP762117GBK
CSB-EP762117GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Bifunctional protein GlmU (glmU) CSB-YP762118GBK
CSB-EP762118GBK
CSB-BP762118GBK
CSB-MP762118GBK
CSB-EP762118GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Dihydrodipicolinate synthase (dapA) CSB-YP762119GBK
CSB-EP762119GBK
CSB-BP762119GBK
CSB-MP762119GBK
CSB-EP762119GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP762120GBK
CSB-EP762120GBK
CSB-BP762120GBK
CSB-MP762120GBK
CSB-EP762120GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Serine--tRNA ligase (serS) CSB-YP762121GBK
CSB-EP762121GBK
CSB-BP762121GBK
CSB-MP762121GBK
CSB-EP762121GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Ribonuclease P protein component (rnpA) CSB-YP762122LAW
CSB-EP762122LAW
CSB-BP762122LAW
CSB-MP762122LAW
CSB-EP762122LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii UPF0246 protein LJ_0535 (LJ_0535) CSB-YP762123LAW
CSB-EP762123LAW
CSB-BP762123LAW
CSB-MP762123LAW
CSB-EP762123LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii DNA ligase (ligA), partial CSB-YP762124LAW
CSB-EP762124LAW
CSB-BP762124LAW
CSB-MP762124LAW
CSB-EP762124LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Deoxyribose-phosphate aldolase (deoC) CSB-YP762125LAW
CSB-EP762125LAW
CSB-BP762125LAW
CSB-MP762125LAW
CSB-EP762125LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii UPF0348 protein LJ_1633 (LJ_1633) CSB-YP762126LAW
CSB-EP762126LAW
CSB-BP762126LAW
CSB-MP762126LAW
CSB-EP762126LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii 50S ribosomal protein L27 (rpmA) CSB-YP762127LAW
CSB-EP762127LAW
CSB-BP762127LAW
CSB-MP762127LAW
CSB-EP762127LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Methionyl-tRNA formyltransferase (fmt) CSB-YP762128LAW
CSB-EP762128LAW
CSB-BP762128LAW
CSB-MP762128LAW
CSB-EP762128LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Uridylate kinase (pyrH) CSB-YP762129LAW
CSB-EP762129LAW
CSB-BP762129LAW
CSB-MP762129LAW
CSB-EP762129LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Protein grpE (grpE) CSB-YP762130LAW
CSB-EP762130LAW
CSB-BP762130LAW
CSB-MP762130LAW
CSB-EP762130LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Probable rRNA maturation factor (LJ_1323) CSB-YP762131LAW
CSB-EP762131LAW
CSB-BP762131LAW
CSB-MP762131LAW
CSB-EP762131LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Dihydroorotase (pyrC) CSB-YP762132LAW
CSB-EP762132LAW
CSB-BP762132LAW
CSB-MP762132LAW
CSB-EP762132LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Lipoprotein signal peptidase (lspA), partial CSB-YP762133LAW1
CSB-EP762133LAW1
CSB-BP762133LAW1
CSB-MP762133LAW1
CSB-EP762133LAW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO (trmFO) CSB-YP762134LAW
CSB-EP762134LAW
CSB-BP762134LAW
CSB-MP762134LAW
CSB-EP762134LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii 6-phosphofructokinase (pfkA) CSB-YP762135LAW
CSB-EP762135LAW
CSB-BP762135LAW
CSB-MP762135LAW
CSB-EP762135LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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