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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 30S ribosomal protein S2 (rpsB) CSB-YP761916LEV
CSB-EP761916LEV
CSB-BP761916LEV
CSB-MP761916LEV
CSB-EP761916LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 50S ribosomal protein L10 (rplJ) CSB-YP761917LEV
CSB-EP761917LEV
CSB-BP761917LEV
CSB-MP761917LEV
CSB-EP761917LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP761918LEV
CSB-EP761918LEV
CSB-BP761918LEV
CSB-MP761918LEV
CSB-EP761918LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Probable spermidine synthase (speE) CSB-YP761919LEV
CSB-EP761919LEV
CSB-BP761919LEV
CSB-MP761919LEV
CSB-EP761919LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Glycine cleavage system H protein (gcvH) CSB-YP761920LEV
CSB-EP761920LEV
CSB-BP761920LEV
CSB-MP761920LEV
CSB-EP761920LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Uncharacterized RNA methyltransferase LIC_10249 (LIC_10249) CSB-YP761921LEV
CSB-EP761921LEV
CSB-BP761921LEV
CSB-MP761921LEV
CSB-EP761921LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Fumarate hydratase class II (fumC) CSB-YP761922LEV
CSB-EP761922LEV
CSB-BP761922LEV
CSB-MP761922LEV
CSB-EP761922LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Peptide chain release factor 1 (prfA) CSB-YP761923LEV
CSB-EP761923LEV
CSB-BP761923LEV
CSB-MP761923LEV
CSB-EP761923LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP761924BQN
CSB-EP761924BQN
CSB-BP761924BQN
CSB-MP761924BQN
CSB-EP761924BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Thymidine kinase (tdk) CSB-YP761925BQN
CSB-EP761925BQN
CSB-BP761925BQN
CSB-MP761925BQN
CSB-EP761925BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP synthase subunit delta (atpH) CSB-YP761926BQN
CSB-EP761926BQN
CSB-BP761926BQN
CSB-MP761926BQN
CSB-EP761926BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Prolipoprotein diacylglyceryl transferase 1 (lgt1), partial CSB-YP761927BQN1
CSB-EP761927BQN1
CSB-BP761927BQN1
CSB-MP761927BQN1
CSB-EP761927BQN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Triosephosphate isomerase (tpiA) CSB-YP761928BQN
CSB-EP761928BQN
CSB-BP761928BQN
CSB-MP761928BQN
CSB-EP761928BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Tyrosine--tRNA ligase 2 (tyrS2) CSB-YP761929BQN
CSB-EP761929BQN
CSB-BP761929BQN
CSB-MP761929BQN
CSB-EP761929BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glucose-6-phosphate isomerase (pgi) CSB-YP761930BQN
CSB-EP761930BQN
CSB-BP761930BQN
CSB-MP761930BQN
CSB-EP761930BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus S-adenosylmethionine synthase (metK) CSB-YP761931BQN
CSB-EP761931BQN
CSB-BP761931BQN
CSB-MP761931BQN
CSB-EP761931BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Tyrosine--tRNA ligase 1 (tyrS1) CSB-YP761932BQN
CSB-EP761932BQN
CSB-BP761932BQN
CSB-MP761932BQN
CSB-EP761932BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0173 metal-dependent hydrolase BCE_4747 (BCE_4747) CSB-YP761933BQN
CSB-EP761933BQN
CSB-BP761933BQN
CSB-MP761933BQN
CSB-EP761933BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus MutS2 protein (mutS2), partial CSB-YP761934BQN
CSB-EP761934BQN
CSB-BP761934BQN
CSB-MP761934BQN
CSB-EP761934BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L21 (rplU) CSB-YP761935BQN
CSB-EP761935BQN
CSB-BP761935BQN
CSB-MP761935BQN
CSB-EP761935BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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