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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Photorhabdus luminescens subsp. laumondii Probable phosphoglycerate mutase GpmB (gpmB) CSB-YP762838PIJ
CSB-EP762838PIJ
CSB-BP762838PIJ
CSB-MP762838PIJ
CSB-EP762838PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus laumondii subsp. laumondii Maltose/maltodextrin transport system permease protein MalF (malF), partial CSB-YP762839PIJ1
CSB-EP762839PIJ1
CSB-BP762839PIJ1
CSB-MP762839PIJ1
CSB-EP762839PIJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Elongation factor Tu 1 (tuf1) CSB-YP762840PIJ
CSB-EP762840PIJ
CSB-BP762840PIJ
CSB-MP762840PIJ
CSB-EP762840PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Protein SlyX (slyX) CSB-YP762841PIJ
CSB-EP762841PIJ
CSB-BP762841PIJ
CSB-MP762841PIJ
CSB-EP762841PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP762842PIJ
CSB-EP762842PIJ
CSB-BP762842PIJ
CSB-MP762842PIJ
CSB-EP762842PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Tyrosine--tRNA ligase 2 (tyrS2) CSB-YP762843PIJ
CSB-EP762843PIJ
CSB-BP762843PIJ
CSB-MP762843PIJ
CSB-EP762843PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Shikimate kinase 1 (aroK) CSB-YP762844PIJ
CSB-EP762844PIJ
CSB-BP762844PIJ
CSB-MP762844PIJ
CSB-EP762844PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii ATPase ravA (ravA), partial CSB-YP762845PIJ
CSB-EP762845PIJ
CSB-BP762845PIJ
CSB-MP762845PIJ
CSB-EP762845PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii ATP synthase subunit delta (atpH) CSB-YP762846PIJ
CSB-EP762846PIJ
CSB-BP762846PIJ
CSB-MP762846PIJ
CSB-EP762846PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 50S ribosomal protein L31 type B (rpmE2) CSB-YP762847PIJ
CSB-EP762847PIJ
CSB-BP762847PIJ
CSB-MP762847PIJ
CSB-EP762847PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum tRNA modification GTPase MnmE (mnmE) CSB-YP762848MWI
CSB-EP762848MWI
CSB-BP762848MWI
CSB-MP762848MWI
CSB-EP762848MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Peptide deformylase (def) CSB-YP762849MWI
CSB-EP762849MWI
CSB-BP762849MWI
CSB-MP762849MWI
CSB-EP762849MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Triosephosphate isomerase (tpiA) CSB-YP762850MWI
CSB-EP762850MWI
CSB-BP762850MWI
CSB-MP762850MWI
CSB-EP762850MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Chaperone protein DnaK (dnaK), partial CSB-YP762851MWI
CSB-EP762851MWI
CSB-BP762851MWI
CSB-MP762851MWI
CSB-EP762851MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum 30S ribosomal protein S2 (rpsB) CSB-YP762852MWI
CSB-EP762852MWI
CSB-BP762852MWI
CSB-MP762852MWI
CSB-EP762852MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Spermidine/putrescine import ATP-binding protein PotA (potA), partial CSB-YP762853MWI
CSB-EP762853MWI
CSB-BP762853MWI
CSB-MP762853MWI
CSB-EP762853MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Segregation and condensation protein B (scpB) CSB-YP762854MWI
CSB-EP762854MWI
CSB-BP762854MWI
CSB-MP762854MWI
CSB-EP762854MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum 50S ribosomal protein L21 (rplU) CSB-YP762855MWI
CSB-EP762855MWI
CSB-BP762855MWI
CSB-MP762855MWI
CSB-EP762855MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Fibronectin-binding protein plpA (plpA), partial CSB-YP762856MWI
CSB-EP762856MWI
CSB-BP762856MWI
CSB-MP762856MWI
CSB-EP762856MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Tyrosine--tRNA ligase (tyrS) CSB-YP762857MWI
CSB-EP762857MWI
CSB-BP762857MWI
CSB-MP762857MWI
CSB-EP762857MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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