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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Neurospora crassa Myosin-1 (myo-1), partial CSB-YP763038NHA
CSB-EP763038NHA
CSB-BP763038NHA
CSB-MP763038NHA
CSB-EP763038NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Chromatin modification-related protein eaf-6 (eaf-6) CSB-YP763039NHA
CSB-EP763039NHA
CSB-BP763039NHA
CSB-MP763039NHA
CSB-EP763039NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa tRNA (uracil-O (2)-)-methyltransferase (trm-44), partial CSB-YP763040NHA
CSB-EP763040NHA
CSB-BP763040NHA
CSB-MP763040NHA
CSB-EP763040NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Ubiquinone biosynthesis protein coq-4, mitochondrial (coq-4) CSB-YP763041NHA
CSB-EP763041NHA
CSB-BP763041NHA
CSB-MP763041NHA
CSB-EP763041NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Mediator of RNA polymerase II transcription subunit 31 (soh-1) CSB-YP763042NHA
CSB-EP763042NHA
CSB-BP763042NHA
CSB-MP763042NHA
CSB-EP763042NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Palmitoyltransferase ERF2 (erf-2), partial CSB-YP763043NHA1
CSB-EP763043NHA1
CSB-BP763043NHA1
CSB-MP763043NHA1
CSB-EP763043NHA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Peptidyl-prolyl cis-trans isomerase H (cyp-3) CSB-YP763044NHA
CSB-EP763044NHA
CSB-BP763044NHA
CSB-MP763044NHA
CSB-EP763044NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Ribosome biogenesis protein nsa-2 (nsa-2) CSB-YP763045NHA
CSB-EP763045NHA
CSB-BP763045NHA
CSB-MP763045NHA
CSB-EP763045NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa COP9 signalosome complex subunit 7a (csn-7a) CSB-YP763046NHA
CSB-EP763046NHA
CSB-BP763046NHA
CSB-MP763046NHA
CSB-EP763046NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Probable acetate kinase (B12J7.040, NCU02712) CSB-YP763047NHA
CSB-EP763047NHA
CSB-BP763047NHA
CSB-MP763047NHA
CSB-EP763047NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Nucleolar GTP-binding protein 2 (nog-2), partial CSB-YP763048NHA
CSB-EP763048NHA
CSB-BP763048NHA
CSB-MP763048NHA
CSB-EP763048NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Cytochrome c oxidase assembly protein cox-16, mitochondrial (cox-16), partial CSB-YP763049NHA
CSB-EP763049NHA
CSB-BP763049NHA
CSB-MP763049NHA
CSB-EP763049NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Encephalitozoon cuniculi Homeobox protein HD-9 (HD-9) CSB-YP763050EKH
CSB-EP763050EKH
CSB-BP763050EKH
CSB-MP763050EKH
CSB-EP763050EKH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Divalent-cation tolerance protein CutA (cutA) CSB-YP763051TNT
CSB-EP763051TNT
CSB-BP763051TNT
CSB-MP763051TNT
CSB-EP763051TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anguilla anguilla Fucolectin CSB-YP763052BZF
CSB-EP763052BZF
CSB-BP763052BZF
CSB-MP763052BZF
CSB-EP763052BZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana alata Ribonuclease S-F11 CSB-YP763053NDF
CSB-EP763053NDF
CSB-BP763053NDF
CSB-MP763053NDF
CSB-EP763053NDF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Loligo vulgaris Diisopropyl-fluorophosphatase CSB-YP763054LIA
CSB-EP763054LIA
CSB-BP763054LIA
CSB-MP763054LIA
CSB-EP763054LIA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Ferritin heavy chain, oocyte isoform CSB-YP763055XBE
CSB-EP763055XBE
CSB-BP763055XBE
CSB-MP763055XBE
CSB-EP763055XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Golgin subfamily A member 5 (golga5), partial CSB-YP763056DIL
CSB-EP763056DIL
CSB-BP763056DIL
CSB-MP763056DIL
CSB-EP763056DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio TATA-box-binding protein (tbp) CSB-YP763057DIL
CSB-EP763057DIL
CSB-BP763057DIL
CSB-MP763057DIL
CSB-EP763057DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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