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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Candida glabrata Transcription factor IWS1 (IWS1) CSB-YP763781CZI
CSB-EP763781CZI
CSB-BP763781CZI
CSB-MP763781CZI
CSB-EP763781CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata 37S ribosomal protein S25, mitochondrial (RSM25) CSB-YP763782CZI
CSB-EP763782CZI
CSB-BP763782CZI
CSB-MP763782CZI
CSB-EP763782CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Mitochondrial intermembrane space import and assembly protein 40 (MIA40), partial CSB-YP763783CZI1
CSB-EP763783CZI1
CSB-BP763783CZI1
CSB-MP763783CZI1
CSB-EP763783CZI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Altered inheritance of mitochondria protein 6 (AIM6) CSB-YP763784CZI
CSB-EP763784CZI
CSB-BP763784CZI
CSB-MP763784CZI
CSB-EP763784CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Diphthamide biosynthesis protein 4 (DPH4) CSB-YP763785CZI
CSB-EP763785CZI
CSB-BP763785CZI
CSB-MP763785CZI
CSB-EP763785CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Ubiquitin-like-conjugating enzyme ATG10 (ATG10) CSB-YP763786CZI
CSB-EP763786CZI
CSB-BP763786CZI
CSB-MP763786CZI
CSB-EP763786CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Histone H2A.Z-specific chaperone CHZ1 (CHZ1) CSB-YP763787CZI
CSB-EP763787CZI
CSB-BP763787CZI
CSB-MP763787CZI
CSB-EP763787CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Protein transport protein SEC24-1 (SEC241), partial CSB-YP763788CZI
CSB-EP763788CZI
CSB-BP763788CZI
CSB-MP763788CZI
CSB-EP763788CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Autophagy-related protein 14 (ATG14) CSB-YP763789CZI
CSB-EP763789CZI
CSB-BP763789CZI
CSB-MP763789CZI
CSB-EP763789CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata AdoMet-dependent rRNA methyltransferase SPB1 (SPB1), partial CSB-YP763790CZI
CSB-EP763790CZI
CSB-BP763790CZI
CSB-MP763790CZI
CSB-EP763790CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata SVP1-like protein 2 (HSV2) CSB-YP763791CZI
CSB-EP763791CZI
CSB-BP763791CZI
CSB-MP763791CZI
CSB-EP763791CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Acetyl-coenzyme A synthetase 2 (ACS2), partial CSB-YP763792CZI
CSB-EP763792CZI
CSB-BP763792CZI
CSB-MP763792CZI
CSB-EP763792CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Pre-mRNA-splicing factor CWC24 (CWC24) CSB-YP763793CZI
CSB-EP763793CZI
CSB-BP763793CZI
CSB-MP763793CZI
CSB-EP763793CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Mitochondrial translation factor ATP22 (ATP22) CSB-YP763794CZI
CSB-EP763794CZI
CSB-BP763794CZI
CSB-MP763794CZI
CSB-EP763794CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP763795BSH
CSB-EP763795BSH
CSB-BP763795BSH
CSB-MP763795BSH
CSB-EP763795BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Malate dehydrogenase (mdh) CSB-YP763796BSH
CSB-EP763796BSH
CSB-BP763796BSH
CSB-MP763796BSH
CSB-EP763796BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP763797BSH
CSB-EP763797BSH
CSB-BP763797BSH
CSB-MP763797BSH
CSB-EP763797BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana ATP synthase subunit beta (atpD), partial CSB-YP763798BSH
CSB-EP763798BSH
CSB-BP763798BSH
CSB-MP763798BSH
CSB-EP763798BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP763799BSH
CSB-EP763799BSH
CSB-BP763799BSH
CSB-MP763799BSH
CSB-EP763799BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Histidine--tRNA ligase (hisS) CSB-YP763800BSH
CSB-EP763800BSH
CSB-BP763800BSH
CSB-MP763800BSH
CSB-EP763800BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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