重組蛋白
重組蛋白質(zhì)是分析生物相互作用的有用工具。在研究實驗室中使用了大量的重組蛋白質(zhì),包括商業(yè)蛋白質(zhì)和在特定研究項目中生成的蛋白質(zhì)。它們在許多應(yīng)用中發(fā)揮著關(guān)鍵作用。
如果您正在尋找具有優(yōu)良質(zhì)量(驗證的生物活性,較低的內(nèi)毒素水平和高純度)的重組蛋白質(zhì)來支持您的研究,您來對地方了。
經(jīng)過16多年的發(fā)展,華美生物已經(jīng)建立了從原核到真核的五大系統(tǒng),用于表達重組蛋白質(zhì)。我們擁有一系列蛋白質(zhì)表達和純化技術(shù),包括基于桿狀病毒外膜蛋白(OMP)的蛋白質(zhì)表達技術(shù),以及膜蛋白表達技術(shù)等。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì),或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的抗體,請查看華美生物蛋白質(zhì)定制服務(wù)。
通過選擇目標名稱的首字母瀏覽產(chǎn)品。
產(chǎn)品名稱 | 貨號 | 規(guī)格 |
---|---|---|
Recombinant Chromobacterium violaceum 30S ribosomal protein S15 (rpsO) | CSB-YP762958CKA CSB-EP762958CKA CSB-BP762958CKA CSB-MP762958CKA CSB-EP762958CKA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Chromobacterium violaceum Ribosomal protein S12 methylthiotransferase RimO (rimO) | CSB-YP762959CKA CSB-EP762959CKA CSB-BP762959CKA CSB-MP762959CKA CSB-EP762959CKA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Chromobacterium violaceum Threonine--tRNA ligase (thrS), partial | CSB-YP762960CKA CSB-EP762960CKA CSB-BP762960CKA CSB-MP762960CKA CSB-EP762960CKA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Chromobacterium violaceum Prolipoprotein diacylglyceryl transferase (lgt), partial | CSB-YP762961CKA1 CSB-EP762961CKA1 CSB-BP762961CKA1 CSB-MP762961CKA1 CSB-EP762961CKA1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Chromobacterium violaceum Chorismate synthase (aroC) | CSB-YP762962CKA CSB-EP762962CKA CSB-BP762962CKA CSB-MP762962CKA CSB-EP762962CKA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Chromobacterium violaceum RNA pyrophosphohydrolase (rppH) | CSB-YP762963CKA CSB-EP762963CKA CSB-BP762963CKA CSB-MP762963CKA CSB-EP762963CKA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Chromobacterium violaceum 3-demethylubiquinone-9 3-methyltransferase (ubiG) | CSB-YP762964CKA CSB-EP762964CKA CSB-BP762964CKA CSB-MP762964CKA CSB-EP762964CKA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Chromobacterium violaceum Adenosylhomocysteinase (ahcY) | CSB-YP762965CKA CSB-EP762965CKA CSB-BP762965CKA CSB-MP762965CKA CSB-EP762965CKA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Chromobacterium violaceum Triosephosphate isomerase (tpiA) | CSB-YP762966CKA CSB-EP762966CKA CSB-BP762966CKA CSB-MP762966CKA CSB-EP762966CKA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Chromobacterium violaceum Lipid A export ATP-binding/permease protein MsbA (msbA), partial | CSB-YP762967CKA1 CSB-EP762967CKA1 CSB-BP762967CKA1 CSB-MP762967CKA1 CSB-EP762967CKA1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Chromobacterium violaceum ATP synthase epsilon chain (atpC) | CSB-YP762968CKA CSB-EP762968CKA CSB-BP762968CKA CSB-MP762968CKA CSB-EP762968CKA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Chromobacterium violaceum UPF0102 protein CV_0654 (CV_0654) | CSB-YP762969CKA CSB-EP762969CKA CSB-BP762969CKA CSB-MP762969CKA CSB-EP762969CKA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Chromobacterium violaceum Histidinol-phosphate aminotransferase (hisC) | CSB-YP762970CKA CSB-EP762970CKA CSB-BP762970CKA CSB-MP762970CKA CSB-EP762970CKA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Chromobacterium violaceum Proline--tRNA ligase (proS), partial | CSB-YP762971CKA CSB-EP762971CKA CSB-BP762971CKA CSB-MP762971CKA CSB-EP762971CKA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Chromobacterium violaceum Agmatinase (speB) | CSB-YP762972CKA CSB-EP762972CKA CSB-BP762972CKA CSB-MP762972CKA CSB-EP762972CKA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Chromobacterium violaceum ATP-dependent protease subunit HslV (hslV) | CSB-YP762973CKA CSB-EP762973CKA CSB-BP762973CKA CSB-MP762973CKA CSB-EP762973CKA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Chromobacterium violaceum Phosphomethylpyrimidine synthase (thiC), partial | CSB-YP762974CKA CSB-EP762974CKA CSB-BP762974CKA CSB-MP762974CKA CSB-EP762974CKA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Chromobacterium violaceum Ribosomal RNA small subunit methyltransferase A (rsmA) | CSB-YP762975CKA CSB-EP762975CKA CSB-BP762975CKA CSB-MP762975CKA CSB-EP762975CKA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Chromobacterium violaceum Phosphoenolpyruvate carboxylase (ppc), partial | CSB-YP762976CKA CSB-EP762976CKA CSB-BP762976CKA CSB-MP762976CKA CSB-EP762976CKA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pseudomonas syringae pv. syringae Effector protein hopAE1 (hopAE1), partial | CSB-YP762977PWE CSB-EP762977PWE CSB-BP762977PWE CSB-MP762977PWE CSB-EP762977PWE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。
第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條:如何選擇合適的表達載體?
表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。
根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022